PMID- 15060614
OWN - NLM
STAT- MEDLINE
DCOM- 20040618
LR  - 20240109
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 47
IP  - 1
DP  - 2004 Feb
TI  - Physical mapping of barley genes using an ultrasensitive fluorescence in situ 
      hybridization technique.
PG  - 179-89
AB  - The primary objective of this study was to elucidate gene organization and to 
      integrate the genetic linkage map for barley (Hordeum vulgare L.) with a physical 
      map using ultrasensitive fluorescence in situ hybridization (FISH) techniques for 
      detecting signals from restriction fragment length polymorphism (RFLP) clones. In 
      the process, a single landmark plasmid, p18S5Shor, was constructed that 
      identified and oriented all seven of the chromosome pairs. Plasmid p18S5Shor was 
      used in all hybridizations. Fourteen cDNA probes selected from the linkage map 
      for barley H. vulgare 'Steptoe' x H. vulgare 'Morex' (Kleinhofs et al. 1993) were 
      mapped using an indirect tyramide signal amplification technique and assigned to 
      a physical location on one or more chromosomes. The haploid barley genome is 
      large and a complete physical map of the genome is not yet available; however, it 
      was possible to integrate the linkage map and the physical locations of these 
      cDNAs. An estimate of the ratio of base pairs to centimorgans was an average of 
      1.5 Mb/cM in the distal portions of the chromosome arms and 89 Mb/cM near the 
      centromere. Furthermore, while it appears that the current linkage maps are well 
      covered with markers along the length of each arm, the physical map showed that 
      there are large areas of the genome that have yet to be mapped.
FAU - Stephens, J L
AU  - Stephens JL
AD  - Department of Bioagricultural Sciences and Pest Management, Colorado State 
      University, Fort Collins, CO 80523, USA. jsteph@lamar.colostate.edu
FAU - Brown, S E
AU  - Brown SE
FAU - Lapitan, N L V
AU  - Lapitan NL
FAU - Knudson, D L
AU  - Knudson DL
LA  - eng
GR  - AI 34337/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - DNA, Complementary/genetics
MH  - Hordeum/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Physical Chromosome Mapping
MH  - Plasmids/genetics
MH  - Polymorphism, Restriction Fragment Length
EDAT- 2004/04/03 05:00
MHDA- 2004/06/24 05:00
CRDT- 2004/04/03 05:00
PHST- 2004/04/03 05:00 [pubmed]
PHST- 2004/06/24 05:00 [medline]
PHST- 2004/04/03 05:00 [entrez]
AID - g03-084 [pii]
AID - 10.1139/g03-084 [doi]
PST - ppublish
SO  - Genome. 2004 Feb;47(1):179-89. doi: 10.1139/g03-084.