PMID- 15061474
OWN - NLM
STAT- MEDLINE
DCOM- 20040629
LR  - 20191026
IS  - 0391-3988 (Print)
IS  - 0391-3988 (Linking)
VI  - 27
IP  - 2
DP  - 2004 Feb
TI  - Development of co-culture system of hepatocytes with bone marrow cells for 
      expression and maintenance of hepatic functions.
PG  - 118-26
AB  - In this study, a co-culture system of hepatocytes and bone marrow cells (BMCs) 
      was developed and characterized for the expression and maintenance of ammonia 
      metabolism and albumin secretion activities. A culture medium supplemented with 
      epidermal growth factor, insulin, L-proline, hydrocortisone and 20% (v/v) 
      heat-inactivated fetal bovine serum was developed. In addition to adhesive bone 
      marrow cells, the co-existence of non-adhesive bone marrow cells was effective in 
      expressing liver-specific functions for at least 3 weeks. On the other hand, 
      experiments with Transwell in which cultured cells were separated by a 
      semi-permeable membrane, suggested that soluble factors secreted by BMCs are the 
      key components in the functional enhancement of cells. Furthermore, direct 
      contact between hepatocytes and BMCs enhanced the formation of spheroids and the 
      expression of liver specific functions. These results indicate that this 
      co-culture system is promising in, for example, bioartificial liver, regenerative 
      medicine, and liver function simulator applications.
FAU - Murakami, S
AU  - Murakami S
AD  - Department of Chemical Engineering, Faculty of Engineering, Graduate School, 
      Kyushu University, Fukuoka, Japan.
FAU - Ijima, H
AU  - Ijima H
FAU - Ono, T
AU  - Ono T
FAU - Kawakami, K
AU  - Kawakami K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Artif Organs
JT  - The International journal of artificial organs
JID - 7802649
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Insulin)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 9DLQ4CIU6V (Proline)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*physiology
MH  - Cattle
MH  - Cell Communication/drug effects/*physiology
MH  - Cell Culture Techniques/*methods
MH  - Culture Media, Conditioned
MH  - Epidermal Growth Factor/pharmacology
MH  - Hepatocytes/*physiology
MH  - Hydrocortisone/pharmacology
MH  - Insulin/pharmacology
MH  - Male
MH  - Models, Animal
MH  - Proline/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Sensitivity and Specificity
EDAT- 2004/04/06 05:00
MHDA- 2004/06/30 05:00
CRDT- 2004/04/06 05:00
PHST- 2004/04/06 05:00 [pubmed]
PHST- 2004/06/30 05:00 [medline]
PHST- 2004/04/06 05:00 [entrez]
AID - 10.1177/039139880402700207 [doi]
PST - ppublish
SO  - Int J Artif Organs. 2004 Feb;27(2):118-26. doi: 10.1177/039139880402700207.