PMID- 15062083
OWN - NLM
STAT- MEDLINE
DCOM- 20041124
LR  - 20190826
IS  - 0969-2126 (Print)
IS  - 0969-2126 (Linking)
VI  - 12
IP  - 4
DP  - 2004 Apr
TI  - Structure of a helically extended SH3 domain of the T cell adapter protein ADAP.
PG  - 603-10
AB  - The adapter protein ADAP (FYB/SLAP-130) provides a critical link between T cell 
      receptor (TCR) signaling and cell adhesion via the activation of integrins. The 
      C-terminal 70 residues of ADAP show homology to SH3 domains; however, conserved 
      residues of the fold are absent. An alignment and annotation of this domain has 
      therefore been elusive. We have solved the three-dimensional structure of the 
      ADAP C-terminal domain by NMR spectroscopy and show that it represents an altered 
      SH3 domain fold. An N-terminal, amphipathic helix makes extensive contacts to 
      residues of the regular SH3 domain fold, and thereby a composite surface with 
      unusual surface properties is created. We propose this SH3 domain variant to be 
      classified as a helically extended SH3 domain (hSH3 domain) and show that the 
      ADAP-hSH3 domain can no longer bind conventional proline-rich peptides.
FAU - Heuer, Katja
AU  - Heuer K
AD  - Protein Engineering Group, Forschungsinstitut fur Molekulare Pharmakologie and 
      Freie Universitat Berlin, Robert-Rossle-Strasse 10, 13125 Berlin, Germany.
FAU - Kofler, Michael
AU  - Kofler M
FAU - Langdon, Grant
AU  - Langdon G
FAU - Thiemke, Katharina
AU  - Thiemke K
FAU - Freund, Christian
AU  - Freund C
LA  - eng
SI  - PDB/1RI9
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Structure
JT  - Structure (London, England : 1993)
JID - 101087697
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (FYB1 protein, human)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/*chemistry/metabolism
MH  - Amino Acid Sequence
MH  - Cell Adhesion/physiology
MH  - Humans
MH  - Magnetic Resonance Spectroscopy
MH  - Molecular Sequence Data
MH  - Protein Structure, Tertiary
MH  - T-Lymphocytes/metabolism
EDAT- 2004/04/06 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/04/06 05:00
PHST- 2003/11/14 00:00 [received]
PHST- 2004/01/12 00:00 [revised]
PHST- 2004/01/12 00:00 [accepted]
PHST- 2004/04/06 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/04/06 05:00 [entrez]
AID - S0969212604000632 [pii]
AID - 10.1016/j.str.2004.02.021 [doi]
PST - ppublish
SO  - Structure. 2004 Apr;12(4):603-10. doi: 10.1016/j.str.2004.02.021.