PMID- 15063068
OWN - NLM
STAT- MEDLINE
DCOM- 20040727
LR  - 20061115
IS  - 0167-7012 (Print)
IS  - 0167-7012 (Linking)
VI  - 57
IP  - 2
DP  - 2004 May
TI  - A simple remedy against artifactual double bands in denaturing gradient gel 
      electrophoresis.
PG  - 279-81
AB  - Denaturant gradient gel electrophoresis (DGGE) is a widely used method for 
      mutation analysis and for studies of microbial diversity. Particular combinations 
      of target gene fragments and primers may give rise to erroneous DGGE profiles. We 
      report on a very straightforward means to eliminate the artifactual 'double 
      bands' that can be encountered in several applications.
FAU - Janse, Ingmar
AU  - Janse I
AD  - NIOO-KNAW, Department of Microbial Ecology, Centre for Limnology, Rijksstraatweg 
      6, 3631 AC Nieuwersluis, The Netherlands. i.janse@nioo.knaw.nl
FAU - Bok, Jasper
AU  - Bok J
FAU - Zwart, Gabriel
AU  - Zwart G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Artifacts
MH  - Cyanobacteria/genetics/isolation & purification
MH  - DNA Fingerprinting/*methods
MH  - DNA, Bacterial/*analysis
MH  - Electrophoresis, Polyacrylamide Gel/*methods
MH  - False Positive Reactions
MH  - Nucleic Acid Denaturation
MH  - Polymerase Chain Reaction/*methods
MH  - *Water Microbiology
EDAT- 2004/04/06 05:00
MHDA- 2004/07/28 05:00
CRDT- 2004/04/06 05:00
PHST- 2003/08/29 00:00 [received]
PHST- 2003/12/03 00:00 [revised]
PHST- 2003/12/11 00:00 [accepted]
PHST- 2004/04/06 05:00 [pubmed]
PHST- 2004/07/28 05:00 [medline]
PHST- 2004/04/06 05:00 [entrez]
AID - S0167701203003543 [pii]
AID - 10.1016/j.mimet.2003.12.006 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2004 May;57(2):279-81. doi: 10.1016/j.mimet.2003.12.006.