PMID- 15063113
OWN - NLM
STAT- MEDLINE
DCOM- 20040601
LR  - 20061115
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 322
IP  - 1
DP  - 2004 Apr 25
TI  - Oligomer synthesis by priming deficient polymerase in hepatitis B virus core 
      particle.
PG  - 22-30
AB  - Hepadnavirus DNA polymerase functions in DNA synthesis and encapsidation, and 
      acts as a primer for minus-strand DNA synthesis. Through protein priming 
      reaction, a short DNA oligomer synthesized from the bulge of epsilon as template 
      is covalently attached to the Tyr residue in the terminal protein (TP) domain of 
      DNA polymerase. Using endogenous polymerase assays and native agarose gel 
      analysis, we detected endogenous polymerase activity in priming-deficient mutant 
      core particles, but not in reverse transcriptase (RT) reaction- or P 
      protein-deficient mutant core particles. In addition, priming-deficient mutant 
      core particles incorporated radiolabeled (32)P-dATP, (32)P-TTP, and (32)P-dGTP, 
      but not (32)P-dCTP. Our results suggest that the priming-deficient mutant P 
      protein has the ability to synthesize oligomers (presumably nascent minus-strand 
      DNA) in the absence of covalent linkage between TP and the first deoxynucleotide. 
      We propose that the priming-deficient mutant may be defective in minus-strand DNA 
      translocation to direct repeat (DR) 1 at the 3' end of pregenomic RNA (pgRNA) 
      that leads to the elongation of minus-strand DNA.
FAU - Kim, Hee-Young
AU  - Kim HY
AD  - Department of Microbiology, Ajou University School of Medicine, Suwon 442-721, 
      South Korea.
FAU - Park, Gil-Soon
AU  - Park GS
FAU - Kim, Eung-Goo
AU  - Kim EG
FAU - Kang, Seung-Hyun
AU  - Kang SH
FAU - Shin, Ho-Joon
AU  - Shin HJ
FAU - Park, Sun
AU  - Park S
FAU - Kim, Kyongmin Hwang
AU  - Kim KH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (DNA, Viral)
RN  - 0 (Gene Products, pol)
RN  - 0 (Oligopeptides)
RN  - 0 (P protein, Hepatitis B virus)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - DNA, Single-Stranded/biosynthesis
MH  - DNA, Viral/biosynthesis
MH  - Gene Products, pol/deficiency/*metabolism
MH  - Hepatitis B virus/*metabolism
MH  - Oligopeptides/*biosynthesis
MH  - RNA-Directed DNA Polymerase/deficiency/*metabolism
MH  - Repetitive Sequences, Nucleic Acid
MH  - Virus Replication
EDAT- 2004/04/06 05:00
MHDA- 2004/06/02 05:00
CRDT- 2004/04/06 05:00
PHST- 2003/09/21 00:00 [received]
PHST- 2003/11/07 00:00 [revised]
PHST- 2004/01/05 00:00 [accepted]
PHST- 2004/04/06 05:00 [pubmed]
PHST- 2004/06/02 05:00 [medline]
PHST- 2004/04/06 05:00 [entrez]
AID - S0042682204000315 [pii]
AID - 10.1016/j.virol.2004.01.009 [doi]
PST - ppublish
SO  - Virology. 2004 Apr 25;322(1):22-30. doi: 10.1016/j.virol.2004.01.009.