PMID- 15063775
OWN - NLM
STAT- MEDLINE
DCOM- 20040601
LR  - 20121115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 317
IP  - 2
DP  - 2004 Apr 30
TI  - Leukemic cell intracellular responses to nanosecond electric fields.
PG  - 421-7
AB  - Intense, nanosecond (ns) pulsed electric fields (PEFs) are known to affect the 
      intracellular structures of cells. The probability of preferentially inducing 
      subcellular effects increases with decreasing pulse length while effects on the 
      plasma membrane are diminished. This has been demonstrated by applying electrical 
      pulses of 60 and 10 ns duration with electric field intensities of up to 6.5 MV/m 
      to HL-60 cells. Using confocal microscopy, PEF-induced changes in the integrity 
      of the plasma membrane and nucleus were measured by recording fluorescence 
      changes with propidium iodide (PI) and acridine orange (AO), respectively. 
      Results suggest that high voltage, nsPEFs target the nucleus and modify cellular 
      functions while plasma membrane effects are delayed and become smaller as pulse 
      duration is shortened. Cell viability was not affected by these pulses. In spite 
      of the high pulsed electric fields, thermal effects can be neglected because of 
      the ultrashort pulse duration. The results suggest application of this ultrashort 
      pulse technology to modulate nuclear structure and function for potential 
      therapeutic benefit.
FAU - Chen, Nianyong
AU  - Chen N
AD  - Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA. 
      n_ychen@hotmail.com
FAU - Schoenbach, Karl H
AU  - Schoenbach KH
FAU - Kolb, Juergen F
AU  - Kolb JF
FAU - James Swanson, R
AU  - James Swanson R
FAU - Garner, Allen L
AU  - Garner AL
FAU - Yang, Jing
AU  - Yang J
FAU - Joshi, Ravindra P
AU  - Joshi RP
FAU - Beebe, Stephen J
AU  - Beebe SJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Pyridazines)
RN  - FU2BGC781U (propildazine)
SB  - IM
MH  - Adaptation, Physiological/physiology/radiation effects
MH  - Biological Transport, Active/radiation effects
MH  - Cell Membrane/*metabolism/*radiation effects/ultrastructure
MH  - Cell Nucleus/*metabolism/*radiation effects/ultrastructure
MH  - Dose-Response Relationship, Radiation
MH  - *Electromagnetic Fields
MH  - Energy Transfer/*physiology
MH  - HL-60 Cells
MH  - Humans
MH  - Intracellular Membranes/metabolism/radiation effects/ultrastructure
MH  - Pyridazines/*pharmacokinetics
MH  - Radiation Dosage
MH  - Radiation Tolerance/physiology
EDAT- 2004/04/06 05:00
MHDA- 2004/06/02 05:00
CRDT- 2004/04/06 05:00
PHST- 2004/03/05 00:00 [received]
PHST- 2004/04/06 05:00 [pubmed]
PHST- 2004/06/02 05:00 [medline]
PHST- 2004/04/06 05:00 [entrez]
AID - S0006291X04005480 [pii]
AID - 10.1016/j.bbrc.2004.03.063 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2004 Apr 30;317(2):421-7. doi: 
      10.1016/j.bbrc.2004.03.063.