PMID- 15064091
OWN - NLM
STAT- MEDLINE
DCOM- 20041005
LR  - 20061115
IS  - 0021-9150 (Print)
IS  - 0021-9150 (Linking)
VI  - 173
IP  - 2
DP  - 2004 Apr
TI  - Detection of Chlamydophila pneumoniae in dendritic cells in atherosclerotic 
      lesions.
PG  - 185-95
AB  - Dendritic cells (DCs) populate atherosclerotic lesions and might be involved in 
      the regulation of immune reactions in atherosclerosis. The present work was 
      undertaken to examine a possible association of DCs with Chlamydophila pneumoniae 
      in human atherosclerotic plaques obtained by endarterectomy. C. pneumoniae was 
      identified in 17 of 60 (28%) atherosclerotic plaques by a combination of 
      immunohistochemistry and polymerase chain reaction (PCR). Double 
      immunohistochemistry identified the presence of C. pneumoniae within S100(+) DCs 
      that were localised predominantly in the deep layer of the intima under the 
      necrotic core. Quantitative analysis showed that there were no differences in the 
      numbers of DCs between C. pneumoniae(+) and C. pneumoniae(-) groups of 
      atherosclerotic specimens. There were also no differences in the expression of 
      Lag-antigen and HLA-DR by DCs between the groups of specimens. Markers of DC 
      activation CD80 and CD86 were absent from both groups of specimens. Flow 
      cytometry analysis of the effects of C. pneumoniae infection on immature 
      monocyte-derived DCs in vitro showed no changes in the expression of CD1a, MHC 
      class II, CD80 and CD86. The results of this study demonstrate that C. pneumoniae 
      might infect DCs within the atherosclerotic intima but whether the presence of C. 
      pneumoniae in DCs affects the intensity of immune reactions in atherosclerosis 
      needs further clarification.
FAU - Bobryshev, Yuri V
AU  - Bobryshev YV
AD  - Surgical Professional Unit, St. Vincent's Hospital, University of New South 
      Wales, Sydney, Australia. ybobryshev@stvincents.com.au
FAU - Cao, Weiping
AU  - Cao W
FAU - Phoon, Meng Chee
AU  - Phoon MC
FAU - Tran, Dinh
AU  - Tran D
FAU - Chow, Vincent T K
AU  - Chow VT
FAU - Lord, Reginald S A
AU  - Lord RS
FAU - Lu, Jinhua
AU  - Lu J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Atherosclerosis
JT  - Atherosclerosis
JID - 0242543
SB  - IM
MH  - Aged
MH  - Arteriosclerosis/*microbiology/*pathology
MH  - Biopsy, Needle
MH  - Case-Control Studies
MH  - Cells, Cultured
MH  - Chlamydia Infections/*diagnosis
MH  - Chlamydophila pneumoniae/*isolation & purification
MH  - Dendritic Cells/microbiology/pathology
MH  - Female
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Probability
MH  - Reference Values
MH  - Risk Assessment
MH  - Sampling Studies
MH  - Sensitivity and Specificity
MH  - Tunica Intima/microbiology/pathology
EDAT- 2004/04/06 05:00
MHDA- 2004/10/06 09:00
CRDT- 2004/04/06 05:00
PHST- 2003/09/22 00:00 [received]
PHST- 2003/11/27 00:00 [revised]
PHST- 2003/12/19 00:00 [accepted]
PHST- 2004/04/06 05:00 [pubmed]
PHST- 2004/10/06 09:00 [medline]
PHST- 2004/04/06 05:00 [entrez]
AID - S0021-9150(03)00568-9 [pii]
AID - 10.1016/j.atherosclerosis.2003.12.028 [doi]
PST - ppublish
SO  - Atherosclerosis. 2004 Apr;173(2):185-95. doi: 
      10.1016/j.atherosclerosis.2003.12.028.