PMID- 15064153
OWN - NLM
STAT- MEDLINE
DCOM- 20041110
LR  - 20181113
IS  - 0091-6765 (Print)
IS  - 0091-6765 (Linking)
VI  - 112
IP  - 5
DP  - 2004 Apr
TI  - Structural changes in gill DNA reveal the effects of contaminants on Puget Sound 
      fish.
PG  - 511-5
AB  - Structural differences were identified in gill DNA from two groups of English 
      sole collected from Puget Sound, Washington, in October 2000. One group was from 
      the industrialized Duwamish River (DR) in Seattle and the other from relatively 
      clean Quartermaster Harbor (QMH). Chemical markers of sediment contamination 
      [e.g., polynuclear aromatic hydrocarbons (PAHs) and polychlorinated biphenyls 
      (PCBs)] established that the DR was substantially more contaminated than QMH. The 
      levels of these chemicals in the sediments of both sites were consistent with 
      levels of cytochrome P450 1A (CYP1A) expression in the gills of English sole from 
      the same sites. Structural differences in gill DNA between the groups were 
      evinced via statistical models of Fourier transform-infrared (FT-IR) spectra. 
      Marked structural damage was found in the gill DNA of the DR fish as reflected in 
      differences in base functional groups (e.g., C-O and NH2) and conformational 
      properties (e.g., arising from perturbations in vertical base stacking 
      interactions). These DNA differences were used to discriminate between the two 
      fish groups through principal components analysis of mean FT-IR spectra. In 
      addition, logistic regression analysis allowed for the development of a "DNA 
      damage index" to assess the effects of contaminants on the gill. The evidence 
      implies that environmental chemicals contribute to the DNA changes in the gill. 
      The damaged DNA is a promising marker for identifying, through gill biopsies, 
      contaminant effects on fish.
FAU - Malins, Donald C
AU  - Malins DC
AD  - Biochemical Oncology Program, Pacific Northwest Research Institute, 720 Broadway, 
      Seattle, WA 98122, USA. dmalins@pnri.org
FAU - Stegeman, John J
AU  - Stegeman JJ
FAU - Anderson, Jack W
AU  - Anderson JW
FAU - Johnson, Paul M
AU  - Johnson PM
FAU - Gold, Jordan
AU  - Gold J
FAU - Anderson, Katie M
AU  - Anderson KM
LA  - eng
GR  - 5P42 ES 07381/ES/NIEHS NIH HHS/United States
GR  - P42 ES 04696/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Environ Health Perspect
JT  - Environmental health perspectives
JID - 0330411
RN  - 0 (Benzofurans)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Polymers)
RN  - 0 (Water Pollutants, Chemical)
RN  - 0 (polychlorodibenzofuran)
RN  - 9007-49-2 (DNA)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Benzofurans/analysis
MH  - Cytochrome P-450 CYP1A1/metabolism
MH  - DNA/chemistry/*drug effects
MH  - Flounder/anatomy & histology/*genetics
MH  - Geologic Sediments/chemistry
MH  - Gills/*drug effects/metabolism/pathology
MH  - Logistic Models
MH  - Polychlorinated Biphenyls/analysis
MH  - Polychlorinated Dibenzodioxins/*analogs & derivatives/analysis
MH  - Polycyclic Aromatic Hydrocarbons/analysis
MH  - Polymers/analysis
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Washington
MH  - Water Pollutants, Chemical/analysis/*toxicity
PMC - PMC1241913
EDAT- 2004/04/06 05:00
MHDA- 2004/11/13 09:00
PMCR- 2004/04/01
CRDT- 2004/04/06 05:00
PHST- 2004/04/06 05:00 [pubmed]
PHST- 2004/11/13 09:00 [medline]
PHST- 2004/04/06 05:00 [entrez]
PHST- 2004/04/01 00:00 [pmc-release]
AID - 10.1289/ehp.6719 [doi]
PST - ppublish
SO  - Environ Health Perspect. 2004 Apr;112(5):511-5. doi: 10.1289/ehp.6719.