PMID- 15064241
OWN - NLM
STAT- MEDLINE
DCOM- 20040517
LR  - 20220227
IS  - 1040-0605 (Print)
IS  - 1040-0605 (Linking)
VI  - 286
IP  - 5
DP  - 2004 May
TI  - Anti-monocyte chemoattractant protein-1 gene therapy attenuates pulmonary 
      fibrosis in mice.
PG  - L1038-44
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a proinflammatory chemokine and may 
      play an important role in the development of pulmonary fibrosis. We examined a 
      new therapeutic strategy that comprises the transfection of the mutant MCP-1 gene 
      into skeletal muscles as a biofactory for anti-MCP-1 therapy against 
      bleomycin-induced pulmonary fibrosis in mice. Overexpression of the mutant MCP-1 
      gene at 10-14 days after intratracheal instillation of bleomycin resulted in 
      decreased DNA damage, apoptosis, and pulmonary fibrosis at 14 days. However, 
      overexpression of the mutant MCP-1 at 0-4 days after bleomycin instillation did 
      not result in decreased pathological grade, DNA damage, or apoptosis at 7 and 14 
      days. Because, in this model, inflammatory cell infiltration begins at 3 days and 
      is followed by interstitial fibrosis, it is likely that MCP-1 has an important 
      role to play in the development of fibrogenesis but not in the development of 
      early lung inflammation. This method does not require the use of viral vector or 
      neutralizing antibody, and, as such, it is possible to avoid problems regarding 
      the pathogenicity of the viral vector or immunocomplex. This new strategy may be 
      a beneficial method of treating pulmonary fibrosis from the viewpoint of clinical 
      application.
FAU - Inoshima, Ichiro
AU  - Inoshima I
AD  - Research Inst. for Diseases of the Chest, Graduate School of Medical Sciences, 
      Kyushu Univ., 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
FAU - Kuwano, Kazuyoshi
AU  - Kuwano K
FAU - Hamada, Naoki
AU  - Hamada N
FAU - Hagimoto, Naoki
AU  - Hagimoto N
FAU - Yoshimi, Michihiro
AU  - Yoshimi M
FAU - Maeyama, Takashige
AU  - Maeyama T
FAU - Takeshita, Akira
AU  - Takeshita A
FAU - Kitamoto, Shiro
AU  - Kitamoto S
FAU - Egashira, Kensuke
AU  - Egashira K
FAU - Hara, Nobuyuki
AU  - Hara N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol Lung Cell Mol Physiol
JT  - American journal of physiology. Lung cellular and molecular physiology
JID - 100901229
RN  - 0 (Chemokine CCL2)
RN  - 11056-06-7 (Bleomycin)
SB  - IM
MH  - Animals
MH  - Bleomycin
MH  - Chemokine CCL2/*genetics
MH  - Disease Models, Animal
MH  - Genetic Therapy/*methods
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscle, Skeletal/physiopathology
MH  - Pulmonary Fibrosis/chemically induced/*therapy
MH  - Transfection
EDAT- 2004/04/06 05:00
MHDA- 2004/05/18 05:00
CRDT- 2004/04/06 05:00
PHST- 2004/04/06 05:00 [pubmed]
PHST- 2004/05/18 05:00 [medline]
PHST- 2004/04/06 05:00 [entrez]
AID - 286/5/L1038 [pii]
AID - 10.1152/ajplung.00167.2003 [doi]
PST - ppublish
SO  - Am J Physiol Lung Cell Mol Physiol. 2004 May;286(5):L1038-44. doi: 
      10.1152/ajplung.00167.2003.