PMID- 15064627
OWN - NLM
STAT- MEDLINE
DCOM- 20040511
LR  - 20051117
IS  - 0023-852X (Print)
IS  - 0023-852X (Linking)
VI  - 114
IP  - 4
DP  - 2004 Apr
TI  - p53-positive tumor-distant squamous epithelia of the head and neck reveal 
      selective loss of chromosome 17.
PG  - 698-704
AB  - OBJECTIVES/HYPOTHESIS: The progression of clinically manifest premalignant 
      lesions in the head and neck region to primary or second primary cancer is 
      characterized by numerical and structural chromosomal aberrations. However, many 
      tumors arise from histologically inconspicuous mucosal sites. The objective was 
      to investigate whether chromosomal aberrations can be detected in tumor-distant 
      mucosa and whether they can help predict the risk of second primary malignancy. 
      STUDY DESIGN: A retrospective series of 72 clinically healthy, arbitrarily taken 
      mucosal samples from 53 patients with squamous cell carcinoma of the head and 
      neck was studied. A previous analysis of the p53 protein status had revealed both 
      p53-positive and p53-negative samples. METHODS: The samples were analyzed by 
      fluorescence in situ hybridization (FISH) using centromeric probes specific for 
      the chromosomes 1, 10, 17, and 18. RESULTS: Tumor-distant mucosa generally showed 
      increased numerical chromosomal aberrations, which consisted mainly of 
      monosomies. In the group of patients with p53-positive epithelia, all aberrations 
      including gains of chromosomes (trisomies, tetrasomies) were more frequent. 
      Monosomy for chromosome 17 was most significantly and selectively enhanced in 
      this group (Wilcoxon Scores [rank sum] test, P =.0002). CONCLUSION: Detectable 
      chromosomal aberrations occur in clinically healthy epithelia in patients at risk 
      for secondary head and neck cancer. Specifically, unbalanced chromosome 17 
      monosomy in conjunction with p53 protein overexpression may constitute a valuable 
      biomarker for progressive "field cancerization."
FAU - Wolf, Christina
AU  - Wolf C
AD  - Molecular Biology Laboratory, Department of Otorhinolaryngology, University 
      Hospital, School of Medicine, Heidelberg, Germany.
FAU - Flechtenmacher, Christa
AU  - Flechtenmacher C
FAU - Dietz, Andreas
AU  - Dietz A
FAU - Weidauer, Hagen
AU  - Weidauer H
FAU - Abel, Ulrich
AU  - Abel U
FAU - Maier, Heinz
AU  - Maier H
FAU - Bosch, Franz X
AU  - Bosch FX
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Laryngoscope
JT  - The Laryngoscope
JID - 8607378
RN  - 0 (DNA Probes)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Aged
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - DNA Probes/genetics
MH  - Epithelium
MH  - Female
MH  - Head and Neck Neoplasms/*genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Middle Aged
MH  - Retrospective Studies
MH  - Tumor Suppressor Protein p53/*genetics
EDAT- 2004/04/06 05:00
MHDA- 2004/05/12 05:00
CRDT- 2004/04/06 05:00
PHST- 2004/04/06 05:00 [pubmed]
PHST- 2004/05/12 05:00 [medline]
PHST- 2004/04/06 05:00 [entrez]
AID - 00005537-200404000-00019 [pii]
AID - 10.1097/00005537-200404000-00019 [doi]
PST - ppublish
SO  - Laryngoscope. 2004 Apr;114(4):698-704. doi: 10.1097/00005537-200404000-00019.