PMID- 15065601
OWN - NLM
STAT- MEDLINE
DCOM- 20040816
LR  - 20191026
IS  - 0262-0898 (Print)
IS  - 0262-0898 (Linking)
VI  - 21
IP  - 1
DP  - 2004
TI  - TNF-alpha promotes progression of peritoneal metastasis as demonstrated using a 
      green fluorescence protein (GFP)-tagged human gastric cancer cell line.
PG  - 39-47
AB  - The mechanisms underlying progression of peritoneal metastasis by gastric cancer 
      after micrometastasis formation remain unclear. In the present study, we 
      investigated metastasis to the abdominal wall peritoneum, one of the major 
      features of peritoneal spread, using a human gastric cancer cell line (GCIY-EGFP) 
      tagged with the green fluorescence protein gene (GFP). This model allows 
      sensitive, specific and sequential observation of metastasis development from the 
      initial deposits to peritoneal carcinomatosis at the end stage. In the initial 
      phase, GCIY-EGFP cells could form micrometastasis selectively on the omentum and 
      mesenterium in a milky spot-dependent manner, but not on abdominal wall 
      peritoneum lacking milky spots until the late stages. In vitro analysis using 
      primary mesothelial cells revealed addition of TNF-alpha to decrease their stress 
      fibers, leading to morphological change followed by exposure of the 
      submesothelial extracellular matrix (ECM) in intercellular gaps. Such TNF-alpha 
      pretreatment was found to enhance attachment of tumor cells to the mesothelial 
      monolayer. When tumor cells were injected into the peritoneal cavity of TNF-alpha 
      pretreated mice, they could metastasize to the abdominal wall peritoneum from the 
      very early stages, resulting in accelerated accumulation of ascites than in 
      TNF-alpha non-pretreatment controls. RT-PCR analysis revealed that tumor cells 
      express cytokines and chemokines, including TNF-alpha. Furthermore, TNF-alpha 
      treatment results in up-regulation of expression of monocyte chemoattractant 
      protein-1 (MCP-1) and IL-8 by mesothelial cells and of TNF-alpha itself by 
      inflammatory leukocytes in the peritoneal cavity. These results suggest that 
      metastasis to the abdominal wall peritoneum occurs as a second step from the 
      first omental metastasis in a milky spot-independent manner and that TNF-alpha 
      derived from tumor cells, mesothelial cells and inflammatory leukocytes in the 
      peritoneal cavity may be involved in the progression of peritoneal metastasis.
FAU - Mochizuki, Yoshinari
AU  - Mochizuki Y
AD  - Department of Gastroenterological Surgery, Aichi Cancer Center Hospital, Nagoya, 
      Japan.
FAU - Nakanishi, Hayao
AU  - Nakanishi H
FAU - Kodera, Yasuhiro
AU  - Kodera Y
FAU - Ito, Seiji
AU  - Ito S
FAU - Yamamura, Yoshitaka
AU  - Yamamura Y
FAU - Kato, Tomoyuki
AU  - Kato T
FAU - Hibi, Kenji
AU  - Hibi K
FAU - Akiyama, Seiji
AU  - Akiyama S
FAU - Nakao, Akimasa
AU  - Nakao A
FAU - Tatematsu, Masae
AU  - Tatematsu M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Clin Exp Metastasis
JT  - Clinical & experimental metastasis
JID - 8409970
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adenocarcinoma/*pathology
MH  - Animals
MH  - Base Sequence
MH  - Cell Adhesion
MH  - Chemokines/genetics
MH  - Cytokines/genetics
MH  - DNA Primers
MH  - Disease Progression
MH  - Epithelium/pathology
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Transplantation
MH  - Peritoneal Neoplasms/*secondary
MH  - RNA, Messenger/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stomach Neoplasms/*pathology
MH  - Tumor Necrosis Factor-alpha/*physiology
EDAT- 2004/04/07 05:00
MHDA- 2004/08/18 05:00
CRDT- 2004/04/07 05:00
PHST- 2004/04/07 05:00 [pubmed]
PHST- 2004/08/18 05:00 [medline]
PHST- 2004/04/07 05:00 [entrez]
AID - 10.1023/b:clin.0000017181.01474.35 [doi]
PST - ppublish
SO  - Clin Exp Metastasis. 2004;21(1):39-47. doi: 10.1023/b:clin.0000017181.01474.35.