PMID- 15066321
OWN - NLM
STAT- MEDLINE
DCOM- 20040630
LR  - 20061115
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 150
IP  - 2
DP  - 2004 Apr 15
TI  - Heterogeneity of structural abnormalities in the 7q31.3 approximately q34 region 
      in myeloid malignancies.
PG  - 136-43
AB  - Abnormalities in the long arm of chromosome 7 are a frequent chromosomal 
      aberration in myeloid disorders. Most studies have focused on the analysis of 
      del(7q), demonstrating the presence of several minimal deleted regions in 7q22 
      approximately q31. By contrast, few studies in myeloid disorders have been 
      devoted to the analysis of translocations, either balanced or unbalanced, 
      involving 7q. In this study, we used fluorescence in situ hybridization (FISH) to 
      characterize the 7q31.3 approximately q34 region (markers D7S480-D7S2227) in 
      patients with deletion or translocation of 7q. A total of 910 cases of myeloid 
      disorders were studied by conventional cytogenetics. Fifty-eight (6%) patients 
      had structural aberrations of 7q. FISH studies were carried out in the 27 
      patients with involvement of 7q31 approximately q34: 14 cases had an acute 
      myelogenous leukemia and 13 cases had a myelodysplastic syndrome. FISH analysis 
      revealed the existence of high complexity in the 7q31.3 approximately q34 region 
      in patients with unbalanced translocations. No breakpoints in 7q31.3 
      approximately q34 were found in the cases with deletion or balanced 
      translocation. Nevertheless, studies of unbalanced translocations showed several 
      breakpoints in markers D7S480-D7S2227, which delineate a commonly altered region. 
      The complexity of 7q rearrangements suggests that a synergy of different genetic 
      factors, rather than the alteration of a single tumor suppressor gene, could be 
      involved in the pathogenesis of del(7q) in myeloid disorders.
FAU - Gonzalez, M Belen
AU  - Gonzalez MB
AD  - Departamento de Hematologia, Hospital Universitario and Centro de Investigacion 
      del Cancer, Universidad de Salamanca-CSIC, Paseo San Vicente 58-182, Salamanca 
      37007, Spain.
FAU - Gutierrez, Norma C
AU  - Gutierrez NC
FAU - Garcia, Juan L
AU  - Garcia JL
FAU - Schoenmakers, Eric F P M
AU  - Schoenmakers EF
FAU - Sole, Francesc
AU  - Sole F
FAU - Calasanz, M Jose
AU  - Calasanz MJ
FAU - San Miguel, Jesus F
AU  - San Miguel JF
FAU - Hernandez, Jesus M
AU  - Hernandez JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - Chromosomes, Human, Pair 7/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myeloid/*genetics
EDAT- 2004/04/07 05:00
MHDA- 2004/07/01 05:00
CRDT- 2004/04/07 05:00
PHST- 2003/05/22 00:00 [received]
PHST- 2003/08/14 00:00 [revised]
PHST- 2003/08/27 00:00 [accepted]
PHST- 2004/04/07 05:00 [pubmed]
PHST- 2004/07/01 05:00 [medline]
PHST- 2004/04/07 05:00 [entrez]
AID - S0165460803003753 [pii]
AID - 10.1016/j.cancergencyto.2003.08.019 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2004 Apr 15;150(2):136-43. doi: 
      10.1016/j.cancergencyto.2003.08.019.