PMID- 15066839
OWN - NLM
STAT- MEDLINE
DCOM- 20040902
LR  - 20240322
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 70
IP  - 4
DP  - 2004 Apr
TI  - Identification, detection, and spatial resolution of Clostridium populations 
      responsible for cellulose degradation in a methanogenic landfill leachate 
      bioreactor.
PG  - 2414-9
AB  - An anaerobic landfill leachate bioreactor was operated with crystalline cellulose 
      and sterile landfill leachate until a steady state was reached. Cellulose 
      hydrolysis, acidogenesis, and methanogenesis were measured. Microorganisms 
      attached to the cellulose surfaces were hypothesized to be the cellulose 
      hydrolyzers. 16S rRNA gene clone libraries were prepared from this attached 
      fraction and also from the mixed fraction (biomass associated with cellulose 
      particles and in the planktonic phase). Both clone libraries were dominated by 
      Firmicutes phylum sequences (100% of the attached library and 90% of the mixed 
      library), and the majority fell into one of five lineages of the clostridia. 
      Clone group 1 (most closely related to Clostridium stercorarium), clone group 2 
      (most closely related to Clostridium thermocellum), and clone group 5 (most 
      closely related to Bacteroides cellulosolvens) comprised sequences in Clostridium 
      group III. Clone group 3 sequences were in Clostridium group XIVa (most closely 
      related to Clostridium sp. strain XB90). Clone group 4 sequences were affiliated 
      with a deeply branching clostridial lineage peripherally associated with 
      Clostridium group VI. This monophyletic group comprises a new Clostridium 
      cluster, designated cluster VIa. Specific fluorescence in situ hybridization 
      (FISH) probes for the five groups were designed and synthesized, and it was 
      demonstrated in FISH experiments that bacteria targeted by the probes for clone 
      groups 1, 2, 4, and 5 were very abundant on the surfaces of the cellulose 
      particles and likely the key cellulolytic microorganisms in the landfill 
      bioreactor. The FISH probe for clone group 3 targeted cells in the planktonic 
      phase, and these organisms were hypothesized to be glucose fermenters.
FAU - Burrell, P C
AU  - Burrell PC
AD  - School of Molecular and Microbial Sciences. School of Engineering, The University 
      of Queensland, St. Lucia 4072, Australia.
FAU - O'Sullivan, C
AU  - O'Sullivan C
FAU - Song, H
AU  - Song H
FAU - Clarke, W P
AU  - Clarke WP
FAU - Blackall, L L
AU  - Blackall LL
LA  - eng
SI  - GENBANK/AY330123
SI  - GENBANK/AY330124
SI  - GENBANK/AY330125
SI  - GENBANK/AY330126
SI  - GENBANK/AY330127
SI  - GENBANK/AY330128
SI  - GENBANK/AY330129
SI  - GENBANK/AY330130
SI  - GENBANK/AY336984
SI  - GENBANK/AY336985
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 9004-34-6 (Cellulose)
SB  - IM
MH  - Base Sequence
MH  - Biomass
MH  - Bioreactors
MH  - Cellulose/*metabolism
MH  - Clostridium/genetics/*isolation & purification/*metabolism
MH  - DNA, Bacterial/genetics
MH  - Genes, Bacterial
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Refuse Disposal
PMC - PMC383074
EDAT- 2004/04/07 05:00
MHDA- 2004/09/03 05:00
PMCR- 2004/04/01
CRDT- 2004/04/07 05:00
PHST- 2004/04/07 05:00 [pubmed]
PHST- 2004/09/03 05:00 [medline]
PHST- 2004/04/07 05:00 [entrez]
PHST- 2004/04/01 00:00 [pmc-release]
AID - 1419 [pii]
AID - 10.1128/AEM.70.4.2414-2419.2004 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2004 Apr;70(4):2414-9. doi: 
      10.1128/AEM.70.4.2414-2419.2004.