PMID- 15067060
OWN - NLM
STAT- MEDLINE
DCOM- 20040727
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 172
IP  - 8
DP  - 2004 Apr 15
TI  - Differential requirement for the CD40-CD154 costimulatory pathway during Th cell 
      priming by CD8 alpha+ and CD8 alpha- murine dendritic cell subsets.
PG  - 4826-33
AB  - Dendritic cells (DCs) regulate the development of distinct Th populations and 
      thereby provoke appropriate immune responses to various kinds of Ags. In the 
      present work, we investigated the role CD40-CD154 interactions play during the 
      process of Th cell priming by CD8 alpha(+) and CD8 alpha(-) murine DC subsets, 
      which have been reported to differently regulate the Th response. Adoptive 
      transfer of Ag-pulsed CD8 alpha(+) DCs induced a Th1 response and the production 
      of IgG2a Abs, whereas transfer of CD8 alpha(-) DCs induced Th2 cells and IgE Abs 
      in vivo. Induction of distinct Th populations by each DC subset was also 
      confirmed in vitro. Although interruption of CD80/CD86-CD28 interactions 
      inhibited Th cell priming by both DC subsets, disruption of CD40-CD154 
      interactions only inhibited the induction of the Th1 response by CD8 alpha(+) DCs 
      in vivo. CD40-CD154 interactions were not required for the proliferation of 
      Ag-specific naive Th cells stimulated by either DC subset, but were indispensable 
      in the production of IL-12 from CD8 alpha(+) DCs and their induction of Th1 cells 
      in vitro. Taken together, in our immunization model of Ag-pulsed DC transfer, 
      CD40-CD154 interactions play an important role in the development of CD8 alpha(+) 
      DC-driven Th1 responses but not CD8 alpha(-) DC-driven Th2 responses to protein 
      Ags.
FAU - Yasumi, Takahiro
AU  - Yasumi T
AD  - Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto, 
      Japan.
FAU - Katamura, Kenji
AU  - Katamura K
FAU - Yoshioka, Takakazu
AU  - Yoshioka T
FAU - Meguro, Taka-aki
AU  - Meguro TA
FAU - Nishikomori, Ryuta
AU  - Nishikomori R
FAU - Heike, Toshio
AU  - Heike T
FAU - Inobe, Manabu
AU  - Inobe M
FAU - Kon, Shigeyuki
AU  - Kon S
FAU - Uede, Toshimitsu
AU  - Uede T
FAU - Nakahata, Tatsutoshi
AU  - Nakahata T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (CD40 Antigens)
RN  - 0 (CD8 Antigens)
RN  - 0 (CD8alpha antigen)
RN  - 0 (Cytokines)
RN  - 147205-72-9 (CD40 Ligand)
SB  - IM
MH  - Animals
MH  - CD40 Antigens/biosynthesis/metabolism/*physiology
MH  - CD40 Ligand/biosynthesis/metabolism/*physiology
MH  - CD8 Antigens/*biosynthesis/metabolism
MH  - Cell Division/immunology
MH  - Cells, Cultured
MH  - Cytokines
MH  - Dendritic Cells/*immunology/*metabolism/transplantation
MH  - Immunophenotyping
MH  - Lymphocyte Activation/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Signal Transduction/*immunology
MH  - T-Lymphocytes, Helper-Inducer/cytology/*immunology/metabolism
MH  - Th1 Cells/immunology
EDAT- 2004/04/07 05:00
MHDA- 2004/07/28 05:00
CRDT- 2004/04/07 05:00
PHST- 2004/04/07 05:00 [pubmed]
PHST- 2004/07/28 05:00 [medline]
PHST- 2004/04/07 05:00 [entrez]
AID - 10.4049/jimmunol.172.8.4826 [doi]
PST - ppublish
SO  - J Immunol. 2004 Apr 15;172(8):4826-33. doi: 10.4049/jimmunol.172.8.4826.