PMID- 15068560
OWN - NLM
STAT- MEDLINE
DCOM- 20040901
LR  - 20190513
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 28
IP  - 2
DP  - 2004 Mar
TI  - Simultaneous determination of eight underivatized amphetamines in hair by 
      high-performance liquid chromatography-atmospheric pressure chemical ionization 
      mass spectrometry (HPLC-APCI-MS).
PG  - 77-85
AB  - The aim of this study was to develop, optimize, and validate an analytical 
      procedure to determine a group of eight amphetamines, amphetamine (AMP), 
      ephedrine (EP), methcathinone (MTC), paramethoxyamphetamine (PMA), 
      methamphetamine (MA), methylenedioxyamphetamine (MDA), 
      methylenedioxymethamphetamine (MDMA), and methylenedioxyethylamphetamine (MDEA), 
      in hair. The target substances were extracted with 1-chlorobutane after alkaline 
      (1M NaOH) digestion and analyzed underivatized with high-performance liquid 
      chromatography-atmospheric pressure chemical ionization mass spectrometry 
      (LC-APCI-MS). APCI-MS parameters such as fragmentor and capillary voltage, corona 
      current, drying gas temperature and flow rate, vaporizer temperature, and 
      nebulizer gas pressure were optimized with the application of flow injection 
      analysis. Quantitation was performed using selected ion monitoring of protonated 
      molecular ions of the studied drugs, and their deuterated analogues were used as 
      internal standards. The limits of detection were 0.05 ng/mg for MA, MDA, MDMA, 
      and MDEA; 0.10 ng/mg for EP and AMP; and 0.20 ng/mg for MTC and PMA. A linear 
      response was observed for all drugs from 0 to 20 ng/mg. The method was applied to 
      the determination of amphetamines in 93 authentic hair samples obtained from 
      detoxification and methadone treatment patients, revealing the following 
      concentrations: 0.17-17.28 ng/mg (EP), 0.52-2.08 ng/mg (MTC), 0.15-62.52 ng/mg 
      (AMP), 0.10-16.52 ng/mg (MA), 0.10-0.88 ng/mg (MDMA), and 0.10-0.16 ng/mg (MDEA). 
      MDA and PMA were never detected.
FAU - Stanaszek, Roman
AU  - Stanaszek R
AD  - Institute of Forensic Research, ul. Westerplatte 9, 31-033 Cracow, Poland. 
      rstanasz@ies.krakow.pl
FAU - Piekoszewski, Wojciech
AU  - Piekoszewski W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Amphetamines)
SB  - IM
MH  - Amphetamines/*analysis
MH  - *Chromatography, High Pressure Liquid
MH  - Forensic Medicine/*methods
MH  - Hair/*chemistry
MH  - Humans
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2004/04/08 05:00
MHDA- 2004/09/02 05:00
CRDT- 2004/04/08 05:00
PHST- 2004/04/08 05:00 [pubmed]
PHST- 2004/09/02 05:00 [medline]
PHST- 2004/04/08 05:00 [entrez]
AID - 10.1093/jat/28.2.77 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2004 Mar;28(2):77-85. doi: 10.1093/jat/28.2.77.