PMID- 15068694
OWN - NLM
STAT- MEDLINE
DCOM- 20040610
LR  - 20171116
IS  - 1547-3287 (Print)
IS  - 1547-3287 (Linking)
VI  - 13
IP  - 1
DP  - 2004 Feb
TI  - Immunophenotypic and functional characterization of cord blood dendritic cells.
PG  - 63-70
AB  - Dendritic cells (DCs) play a pivotal role in the activation of T cells, which are 
      effector cells in graft-versus-host disease (GVHD). A low incidence of GVHD 
      following cord blood (CB) transplantation has long been reported; despite this, 
      little information is currently available on the characteristics of CB DCs. The 
      goal of the present study was to investigate the immunophenotypic characteristics 
      and distribution of CB DCs and their subsets. For that purpose we have analyzed 
      15 CB samples as compared to normal peripheral blood (PB) (n = 7) and blood from 
      patients submitted to an allogeneic PB stem cell transplantation (allo-PBSCT) (n 
      = 6). Our results show an overall decreased frequency of DCs in CB due to the 
      presence of significantly lower numbers of CD123inter./CD33inter./CD16+ DCs. 
      Phenotypically, CB DCs displayed a tendency to express lower levels of the 
      gamma-chain interleukin-2 (IL-2) receptor (CD132) and of the CD86 co-stimulatory 
      molecule, supporting a higher degree of immaturity for CB as compared to PB DCs. 
      After activation of CB DCs with lipopolysaccharide (LPS) and interferon-gamma 
      (IFN-gamma) higher frequencies of cytokine-producing cells were found among 
      CD123inter./CD33inter./CD16+ and CD123dim/CD33bright/CD16- DCs; however, when 
      only the cytokine-producing DCs were considered, a significant decrease in the 
      amount of different cytokine (e.g., IL-1beta and IL-6) produced per cell was 
      observed especially for CD16+ CB DCs. These findings support a higher degree of 
      immaturity for CB as compared to PB DCs that might contribute to explain, at 
      least in part, the low incidence and severity of GVHD observed after CB 
      transplantation.
FAU - Crespo, Ines
AU  - Crespo I
AD  - Histocompatibility Centre of Coimbra, Edificio Sao Jeronimo, Praceta Mota Pinto, 
      3030 Coimbra, Portugal.
FAU - Paiva, Artur
AU  - Paiva A
FAU - Couceiro, Anabela
AU  - Couceiro A
FAU - Pimentel, Pedro
AU  - Pimentel P
FAU - Orfao, Alberto
AU  - Orfao A
FAU - Regateiro, Fernando
AU  - Regateiro F
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD33 protein, human)
RN  - 0 (CD86 protein, human)
RN  - 0 (Cytokines)
RN  - 0 (IL3RA protein, human)
RN  - 0 (Interleukin-3 Receptor alpha Subunit)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, IgG)
RN  - 0 (Receptors, Interleukin-2)
RN  - 0 (Receptors, Interleukin-3)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 3)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antigens, CD/biosynthesis
MH  - Antigens, Differentiation, Myelomonocytic/biosynthesis
MH  - B7-2 Antigen
MH  - Cell Lineage
MH  - Cell Separation
MH  - Child
MH  - Child, Preschool
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*cytology
MH  - Female
MH  - Fetal Blood/*cytology
MH  - Flow Cytometry
MH  - Graft vs Host Disease/immunology
MH  - Humans
MH  - Immunophenotyping/*methods
MH  - Infant
MH  - Infant, Newborn
MH  - Interleukin-3 Receptor alpha Subunit
MH  - Male
MH  - Membrane Glycoproteins/biosynthesis
MH  - Middle Aged
MH  - Phenotype
MH  - Receptors, IgG/biosynthesis
MH  - Receptors, Interleukin-2/metabolism
MH  - Receptors, Interleukin-3/biosynthesis
MH  - Sialic Acid Binding Ig-like Lectin 3
MH  - T-Lymphocytes/metabolism
EDAT- 2004/04/08 05:00
MHDA- 2004/06/21 10:00
CRDT- 2004/04/08 05:00
PHST- 2004/04/08 05:00 [pubmed]
PHST- 2004/06/21 10:00 [medline]
PHST- 2004/04/08 05:00 [entrez]
AID - 10.1089/154732804773099263 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2004 Feb;13(1):63-70. doi: 10.1089/154732804773099263.