PMID- 15068696
OWN - NLM
STAT- MEDLINE
DCOM- 20040610
LR  - 20171116
IS  - 1547-3287 (Print)
IS  - 1547-3287 (Linking)
VI  - 13
IP  - 1
DP  - 2004 Feb
TI  - Evaluation of hematopoietic potential generated by transplantation of 
      muscle-derived stem cells in mice.
PG  - 83-92
AB  - Muscle tissue of adult mice has been shown to contain stem cells with 
      hematopoietic repopulation ability in vivo. To determine the functional 
      characteristics of stem cells giving rise to this hematopoietic activity, we have 
      performed hematopoietic reconstitution experiments by the use of muscle versus 
      marrow transplantation in lethally irradiated mice and followed the fate of 
      transplanted cells by Y-chimerism using PCR and fluorescence in situ 
      hybridization (FISH) analysis. We report here that transplantation of murine 
      muscle generate a major hematopoietic chimerism at the level of CFU-C, CFU-S, and 
      terminally-differentiated cells in three generations of lethally irradiated mice 
      followed up to 1 year after transplantation. This potential is totally abolished 
      when muscle grafts were performed by the use of muscle from previously irradiated 
      mice. As compared to marrow transplantation, muscle transplants were able to 
      generate similar potencies to give rise to myeloid, T, B, and natural killer (NK) 
      cells. Interestingly, marrow stem cells that have been generated in primary and 
      then in secondary recipients were able to contribute efficiently to myofibers in 
      the muscle tissue of tertiary recipients. Altogether, our data demonstrate that 
      muscle-derived stem cells present a major hematopoietic repopulating ability with 
      evidence of self-replication in vivo. They are radiation-sensitive and similar to 
      marrow-derived stem cells in terms of their ability to generate multilineage 
      hematopoiesis. Finally, our data demonstrate that muscle-derived hematopoietic 
      stem cells do not lose their ability to contribute to myofiber generation after 
      at least two rounds of serial transplantation, suggesting a potential that is 
      probably equivalent to that generated by marrow transplantation.
FAU - Farace, Francoise
AU  - Farace F
AD  - Translational Research-Cell Therapy Laboratory, Department of Clinical Biology, 
      Institut Gustave Roussy, Villejuif, France.
FAU - Prestoz, Laetitita
AU  - Prestoz L
FAU - Badaoui, Sabrina
AU  - Badaoui S
FAU - Guillier, Martine
AU  - Guillier M
FAU - Haond, Celine
AU  - Haond C
FAU - Opolon, Paule
AU  - Opolon P
FAU - Thomas, Jean-Leon
AU  - Thomas JL
FAU - Zalc, Bernard
AU  - Zalc B
FAU - Vainchenker, William
AU  - Vainchenker W
FAU - Turhan, Ali G
AU  - Turhan AG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/cytology
MH  - Bone Marrow Cells/cytology
MH  - Bone Marrow Transplantation
MH  - Cell Division
MH  - *Cell Lineage
MH  - Cell Separation
MH  - Cell Transplantation
MH  - Chimera
MH  - Female
MH  - Flow Cytometry
MH  - Hematopoiesis
MH  - Hematopoietic Stem Cells/*cytology
MH  - Hematopoietic System
MH  - In Situ Hybridization, Fluorescence
MH  - Killer Cells, Natural/cytology/metabolism
MH  - Leukocyte Common Antigens/biosynthesis
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscles/*cytology/metabolism
MH  - Polymerase Chain Reaction
MH  - Sex Factors
MH  - Stem Cells/metabolism
MH  - T-Lymphocytes/cytology
EDAT- 2004/04/08 05:00
MHDA- 2004/06/21 10:00
CRDT- 2004/04/08 05:00
PHST- 2004/04/08 05:00 [pubmed]
PHST- 2004/06/21 10:00 [medline]
PHST- 2004/04/08 05:00 [entrez]
AID - 10.1089/154732804773099281 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2004 Feb;13(1):83-92. doi: 10.1089/154732804773099281.