PMID- 15070887
OWN - NLM
STAT- MEDLINE
DCOM- 20041207
LR  - 20091119
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 19
IP  - 5
DP  - 2004 May
TI  - The combination of polar body and embryo biopsy does not affect embryo viability.
PG  - 1163-9
AB  - BACKGROUND: The biopsy of both polar bodies and a blastomere from the same embryo 
      was investigated as an approach aimed at increasing the quantity of DNA available 
      for genetic analysis in preimplantation embryos. METHODS: In 113 cycles, 
      preimplantation genetic diagnosis (PGD) was performed for aneuploidy: 19 cycles 
      underwent polar body biopsy, 32 cycles had both polar body and blastomere biopsy 
      done, and the remaining 62 cycles underwent blastomere biopsy. The chromosomal 
      analysis was performed in a two-round fluorescence in situ hybridization (FISH) 
      protocol with probes specific for the chromosomes X, Y, 13, 15, 16, 18, 21 and 
      22. RESULTS: The morphological evaluation of the analysed embryos demonstrated 
      similar rates of development irrespective of the biopsy procedure. Accordingly, 
      the implantation rate did not differ significantly in the three biopsy groups and 
      was 15% after polar body biopsy, 26% after the combined biopsy procedures of 
      polar bodies and blastomeres, and 25% after blastomere biopsy. CONCLUSIONS: The 
      removal of a blastomere subsequent to polar body biopsy does not seem to have 
      negative effects on embryo viability. This approach could be especially valuable 
      for a combined diagnosis of aneuploidy and single-gene disorders in 
      preimplantation embryos generated by couples at high reproductive risk.
FAU - Magli, M Cristina
AU  - Magli MC
AD  - S.I.S.Me.R., Reproductive Medicine Unit, Via Mazzini 12, 40138 Bologna, Italy.
FAU - Gianaroli, Luca
AU  - Gianaroli L
FAU - Ferraretti, Anna P
AU  - Ferraretti AP
FAU - Toschi, Marco
AU  - Toschi M
FAU - Esposito, Francesca
AU  - Esposito F
FAU - Fasolino, M Carmen
AU  - Fasolino MC
LA  - eng
PT  - Journal Article
DEP - 20040407
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
SB  - IM
MH  - Adult
MH  - Aneuploidy
MH  - Biopsy/*adverse effects
MH  - Blastomeres/*cytology
MH  - *Embryonic Development
MH  - Female
MH  - Genetic Testing
MH  - Humans
MH  - Pregnancy
MH  - *Pregnancy Outcome
MH  - Preimplantation Diagnosis/*adverse effects
MH  - Reproductive Techniques, Assisted
EDAT- 2004/04/09 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/04/09 05:00
PHST- 2004/04/09 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/04/09 05:00 [entrez]
AID - deh162 [pii]
AID - 10.1093/humrep/deh162 [doi]
PST - ppublish
SO  - Hum Reprod. 2004 May;19(5):1163-9. doi: 10.1093/humrep/deh162. Epub 2004 Apr 7.