PMID- 1507169
OWN - NLM
STAT- MEDLINE
DCOM- 19920918
LR  - 20190509
IS  - 0449-3060 (Print)
IS  - 0449-3060 (Linking)
VI  - 33 Suppl
DP  - 1992 Mar
TI  - Automated detection of radiation-induced chromosome aberrations following 
      fluorescence in situ hybridization.
PG  - 189-205
AB  - The cytogenetic detection of rare chromosome aberrations induced by ionizing 
      radiation requires the evaluation of large numbers of cells. In this report, an 
      image analysis program based on thresholding of grey level histograms is 
      described for a rapid automated detection of chromosome translocations in 
      metaphase spreads from human lymphocytes following irradiation and chromosome in 
      situ suppression (CISS) fluorescence in situ hybridization (FISH). To classify a 
      metaphase spread as "normal", "aberrant", or "excluded", a minimum medium time of 
      less than two seconds was required on a general purpose Personal Computer. Using 
      appropriately stained specimen, for the false classification rate an upper limit 
      of about 10% was estimated. The upper limit for the rate of "excluded" cells was 
      estimated to be about 11% (99% confidence ranges). FISH-procedures allow to score 
      chromosome aberrations also in the interphase nucleus. To apply simple threshold 
      algorithms for segmentation of the FISH-stained nuclear areas, it is required 
      that the grey level contrast is sufficiently high. The preliminary results 
      presented here on the image analysis of human lymphocyte nuclei suggest the 
      feasibility of such an approach.
FAU - Cremer, C
AU  - Cremer C
AD  - Institute of Applied Physics, University Heidelberg, Germany.
FAU - Remm, B
AU  - Remm B
FAU - Bischoff, A
AU  - Bischoff A
FAU - Vollweiler, T
AU  - Vollweiler T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Radiat Res
JT  - Journal of radiation research
JID - 0376611
SB  - IM
MH  - Chromosomes/*radiation effects
MH  - Fluorescence
MH  - Humans
MH  - *Image Processing, Computer-Assisted
MH  - Lymphocytes/radiation effects/*ultrastructure
MH  - *Nucleic Acid Hybridization
MH  - Translocation, Genetic/*genetics
EDAT- 1992/03/01 00:00
MHDA- 1992/03/01 00:01
CRDT- 1992/03/01 00:00
PHST- 1992/03/01 00:00 [pubmed]
PHST- 1992/03/01 00:01 [medline]
PHST- 1992/03/01 00:00 [entrez]
AID - 10.1269/jrr.33.supplement_189 [doi]
PST - ppublish
SO  - J Radiat Res. 1992 Mar;33 Suppl:189-205. doi: 10.1269/jrr.33.supplement_189.