PMID- 15077020
OWN - NLM
STAT- MEDLINE
DCOM- 20040505
LR  - 20190713
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 77
IP  - 6
DP  - 2004 Mar 27
TI  - A delay in bone marrow transplantation after partial conditioning improves 
      engraftment.
PG  - 819-26
AB  - BACKGROUND: In the present study we examined the effect of the timing of marrow 
      infusion on engraftment in nonmyeloablatively conditioned mice. METHODS: B10 mice 
      were conditioned with decreasing doses of total body irradiation (TBI) and 
      reconstituted with bone marrow cells (BMCs) from major histocompatibility 
      complex-disparate donor B10.BR mice at 0 or 6 hr, or on days 1, 2, 3, 4, 5, 8, 
      and 12 with respect to TBI. RESULTS: After undergoing conditioning with 700 cGy 
      TBI and transplantation with 15 x 10(6) BMCs, 100% of recipients engrafted if the 
      marrow was infused between 0 and 4 days after TBI. For lower doses of TBI, a 
      delay in infusion of the marrow after TBI conditioning was associated with a 
      significant increase in engraftment. Significantly less engraftment was achieved 
      in animals conditioned with 600 cGy TBI if the marrow was infused at 0 or 6 hr 
      compared with a 1- to 4-day delay. When the TBI was decreased to 500 cGy, 
      engraftment occurred only when the transplant was performed between days 2 and 8. 
      The highest proportion of recipients engrafted when the marrow was infused on day 
      4. This enhanced engraftment after a delay in marrow infusion is associated with 
      a significant reduction in host mixed lymphocyte reaction reactivity and is 
      correlated inversely with serum levels of interleukin-6 in the recipient. 
      CONCLUSIONS: These data demonstrate for the first time that a delay between 
      conditioning and marrow infusion significantly improves allogeneic engraftment in 
      nonmyeloablatively conditioned recipients and reduces the total conditioning 
      required.
FAU - Xu, Hong
AU  - Xu H
AD  - Institute for Cellular Therapeutics, University of Louisville, Kentucky 40202, 
      USA.
FAU - Exner, Beate G
AU  - Exner BG
FAU - Chilton, Paula M
AU  - Chilton PM
FAU - Tanner, Michael K
AU  - Tanner MK
FAU - Mueller, Yvonne M
AU  - Mueller YM
FAU - Rezzoug, Francine
AU  - Rezzoug F
FAU - Ildstad, Suzanne T
AU  - Ildstad ST
LA  - eng
GR  - DK43901/DK/NIDDK NIH HHS/United States
GR  - NHLBI 63442/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/immunology/methods/*physiology
MH  - Graft Rejection
MH  - Islets of Langerhans/cytology
MH  - Islets of Langerhans Transplantation/methods/physiology
MH  - Lymphocyte Culture Test, Mixed
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred Strains
MH  - Transplantation Chimera
MH  - Transplantation, Homologous/physiology
MH  - Whole-Body Irradiation
EDAT- 2004/04/13 05:00
MHDA- 2004/05/07 05:00
CRDT- 2004/04/13 05:00
PHST- 2004/04/13 05:00 [pubmed]
PHST- 2004/05/07 05:00 [medline]
PHST- 2004/04/13 05:00 [entrez]
AID - 00007890-200403270-00006 [pii]
AID - 10.1097/01.tp.0000116414.66171.81 [doi]
PST - ppublish
SO  - Transplantation. 2004 Mar 27;77(6):819-26. doi: 
      10.1097/01.tp.0000116414.66171.81.