PMID- 15077035
OWN - NLM
STAT- MEDLINE
DCOM- 20040505
LR  - 20190713
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 77
IP  - 6
DP  - 2004 Mar 27
TI  - Critical evaluation of the amino acid triplet-epitope matching concept in cadaver 
      kidney transplantation.
PG  - 902-7
AB  - BACKGROUND: A computer-based approach for determining human leukocyte antigen 
      (HLA) compatibility between kidney donors and recipients on the basis of 
      differences of amino acid sequences as motifs for immunogenic epitopes was 
      proposed by Duquesnoy et al. The HLAMatchmaker algorithm focuses on HLA class I 
      polymorphisms of serologically defined antigens encoded by the HLA-A and -B loci. 
      HLA phenotypic mismatches that represent only a few mismatches at the amino acid 
      triplet level are held to be not or only mildly immunogenic. This approach was 
      proposed as being especially suitable for the allocation of donor kidneys to 
      highly sensitized patients. METHODS: We reexamined this attractive concept using 
      the data of the Collaborative Transplant Study. Intra- and interlocus comparisons 
      for HLA-A and -B were performed according to the original HLAMatchmaker 
      algorithm. To exclude the influence of HLA-DR, only transplants with no HLA-DR 
      mismatch were considered. Patients who had one HLA-A and one HLA-B antigen 
      mismatch were separated into subgroups, depending on the number of triplet 
      mismatches as calculated by the HLAMatchmaker software. Separate analyses were 
      performed for first transplants, retransplants, and patients with a 
      panel-reactive antibody activity of 50% or more. A total of 16,997 white patients 
      matched for HLA-DR who received a cadaver kidney transplant between 1991 and 2001 
      formed the basis of this analysis. RESULTS: Application of the HLAMatchmaker 
      method could not be shown to result in any statistically significant effect on 
      graft survival. CONCLUSIONS: The HLAMatchmaker concept is theoretically 
      attractive; however, it could not be shown to yield useful results in this 
      analysis. Serologic HLA typing appears to provide an insufficient basis for 
      applying epitope matching in clinical kidney transplantation.
FAU - Laux, Gunter
AU  - Laux G
AD  - Department of Transplantation Immunology, Institute of Immunology, University of 
      Heidelberg, Heidelberg, Germany. gunter.laux@med.uni-heidelberg.de
FAU - Mytilineos, Joannis
AU  - Mytilineos J
FAU - Opelz, Gerhard
AU  - Opelz G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (Oligopeptides)
RN  - 0 (Peptide Fragments)
SB  - IM
CIN - Transplantation. 2004 Mar 27;77(6):801-2. PMID: 15077016
CIN - Transplantation. 2005 Jan 27;79(2):250-1. PMID: 15665779
MH  - Algorithms
MH  - Amino Acid Sequence
MH  - Cadaver
MH  - HLA-A Antigens/immunology
MH  - HLA-B Antigens/immunology
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Kidney Transplantation/*immunology
MH  - Major Histocompatibility Complex
MH  - Oligopeptides/chemistry/immunology
MH  - Peptide Fragments/chemistry/immunology
MH  - Polymorphism, Genetic
MH  - Tissue Donors
EDAT- 2004/04/13 05:00
MHDA- 2004/05/07 05:00
CRDT- 2004/04/13 05:00
PHST- 2004/04/13 05:00 [pubmed]
PHST- 2004/05/07 05:00 [medline]
PHST- 2004/04/13 05:00 [entrez]
AID - 00007890-200403270-00021 [pii]
AID - 10.1097/01.tp.0000114595.59168.3b [doi]
PST - ppublish
SO  - Transplantation. 2004 Mar 27;77(6):902-7. doi: 10.1097/01.tp.0000114595.59168.3b.