PMID- 15078551
OWN - NLM
STAT- MEDLINE
DCOM- 20040524
LR  - 20181130
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 19
IP  - 7
DP  - 2004 Apr
TI  - Modulation of photic resetting in rats by lesions of projections to the 
      suprachiasmatic nuclei expressing p75 neurotrophin receptor.
PG  - 1773-88
AB  - The suprachiasmatic nuclei of the hypothalamus (SCN) are the site of the master 
      circadian clock in mammals. The SCN clock is mainly entrained by the light-dark 
      cycle. Light information is conveyed from the retina to the SCN through direct, 
      retinohypothalamic fibres. The SCN also receive other projections, like 
      cholinergic fibres from basal forebrain. To test whether cholinergic afferents 
      are involved in photic resetting, lesions of cholinergic projections were 
      performed in rats with intracerebroventricular (i.c.v.) injections or intra-SCN 
      microinjections of 192 IgG-saporin. When injected in the SCN, this immunotoxin 
      destroys the cholinergic projections and retinohypothalamic afferents that 
      express p75 low-affinity nerve growth factor (p75(NGF)) receptors. The extent of 
      lesions in the basal forebrain and SCN was assessed by acetylcholinesterase 
      histochemistry, p75(NGF) receptor, choline acetyl-transferase, calbindin-D28K and 
      VIP immunocytochemistry. The intra-SCN treatment reduced light-induced phase 
      advances by 30%, and induced a complete loss of forebrain and retinal afferents 
      expressing p75(NGF) receptors within the SCN and a decrease of forebrain 
      cholinergic neurons, most likely those projecting to the SCN. The i.c.v. 
      treatment reduced light-induced phase advances by 40%, increased phase delays and 
      led to extensive damage of forebrain p75(NGF)-expressing neurons, while sparing 
      half of the fibres expressing p75(NGF) receptors (retinal afferents?) in the SCN. 
      Because the integrity of forebrain p75(NGF)-expressing neurons appears to be 
      critical in mediating the effects on light-induced phase advances, we therefore 
      suggest that anterior cholinergic projections expressing p75(NGF) receptors 
      modulate the sensitivity of the SCN clock to the phase advancing effects of 
      light.
FAU - Erhardt, Christine
AU  - Erhardt C
AD  - Laboratory of Neurobiology of Rhythms, Centre National de la Recherche 
      Scientifique (UMR7518), Department of Neuroscience (IFR37), University Louis 
      Pasteur, 12 rue de l'universite, 67000 Strasbourg, France.
FAU - Galani, Rodrigue
AU  - Galani R
FAU - Jeltsch, Helene
AU  - Jeltsch H
FAU - Cassel, Jean-Christophe
AU  - Cassel JC
FAU - Klosen, Paul
AU  - Klosen P
FAU - Menet, Jerome S
AU  - Menet JS
FAU - Pevet, Paul
AU  - Pevet P
FAU - Challet, Etienne
AU  - Challet E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (192 IgG-saporin)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Calb1 protein, rat)
RN  - 0 (Calbindin 1)
RN  - 0 (Calbindins)
RN  - 0 (Immunotoxins)
RN  - 0 (Receptor, Nerve Growth Factor)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 0 (Ribosome Inactivating Proteins, Type 1)
RN  - 0 (S100 Calcium Binding Protein G)
RN  - 37221-79-7 (Vasoactive Intestinal Peptide)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
RN  - EC 3.2.2.- (N-Glycosyl Hydrolases)
RN  - EC 3.2.2.22 (Saporins)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/metabolism
MH  - Acetylcholinesterase/metabolism
MH  - Animals
MH  - Antibodies, Monoclonal/toxicity
MH  - Body Temperature/drug effects
MH  - Brain Diseases/metabolism/physiopathology
MH  - Calbindin 1
MH  - Calbindins
MH  - Cell Count/methods
MH  - Cholinergic Fibers/drug effects/*metabolism
MH  - Circadian Rhythm/drug effects/*physiology
MH  - Denervation
MH  - Drug Administration Routes
MH  - Immunohistochemistry/methods
MH  - Immunotoxins/toxicity
MH  - *Light
MH  - Male
MH  - Medial Forebrain Bundle
MH  - Motor Activity/drug effects
MH  - N-Glycosyl Hydrolases
MH  - Prosencephalon/drug effects/metabolism
MH  - Psychomotor Performance/drug effects
MH  - Rats
MH  - Rats, Long-Evans
MH  - Receptor, Nerve Growth Factor
MH  - Receptors, Nerve Growth Factor/*metabolism/radiation effects
MH  - Ribosome Inactivating Proteins, Type 1
MH  - S100 Calcium Binding Protein G/metabolism
MH  - Saporins
MH  - Staining and Labeling/methods
MH  - Suprachiasmatic Nucleus/*metabolism/pathology
MH  - Time Factors
MH  - Vasoactive Intestinal Peptide/metabolism
EDAT- 2004/04/14 05:00
MHDA- 2004/05/25 05:00
CRDT- 2004/04/14 05:00
PHST- 2004/04/14 05:00 [pubmed]
PHST- 2004/05/25 05:00 [medline]
PHST- 2004/04/14 05:00 [entrez]
AID - EJN3281 [pii]
AID - 10.1111/j.1460-9568.2004.03281.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2004 Apr;19(7):1773-88. doi: 10.1111/j.1460-9568.2004.03281.x.