PMID- 15078943
OWN - NLM
STAT- MEDLINE
DCOM- 20040601
LR  - 20190509
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 78
IP  - 9
DP  - 2004 May
TI  - Discovery of a second form of tripartite complex containing gH-gL of human 
      herpesvirus 6 and observations on CD46.
PG  - 4609-16
AB  - The human herpesvirus 6 (HHV-6) glycoprotein H (gH)-glycoprotein L (gL) complex 
      associates with glycoprotein Q (gQ) (Y. Mori, P. Akkapaiboon, X. Yang, and K. 
      Yamanishi, J. Virol. 77:2452-2458, 2003), and the gH-gL-gQ complex interacts with 
      human CD46 (Y. Mori, X. Yang, P. Akkapaiboon, T. Okuno, and K. Yamanishi, J. 
      Virol. 77:4992-4999, 2003). Here, we show that the HHV-6 U47 gene, which is a 
      positional homolog of the human cytomegalovirus glycoprotein O (gO) gene, encodes 
      a third component of the HHV-6 gH-gL-containing envelope complex. A monoclonal 
      antibody (MAb) against the amino terminus of HHV-6 gO reacted in immunoblots with 
      protein species migrating at 120 to 130 kDa and 74 to 80 kDa in lysates of 
      HHV-6-infected cells and with a 74- to 80-kDa protein species in purified 
      virions. The 80-kDa form of gO was coimmunoprecipitated with an anti-gH MAb, but 
      an anti-gQ MAb, which coimmunoprecipitated gH, did not coprecipitate gO. 
      Furthermore, the gH-gL-gO complex did not bind to human CD46, indicating that the 
      complex was not a ligand for CD46. These findings suggested that the viral 
      envelope contains at least two kinds of tripartite complexes, gH-gL-gQ and 
      gH-gL-gO, and that the gH-gL-gO complex may play a role different from that of 
      gH-gL-gQ during viral infection. This is the first report of two kinds of gH-gL 
      complexes on the viral envelope in a member of the herpesvirus family.
FAU - Mori, Yasuko
AU  - Mori Y
AD  - Department of Microbiology, Graduate School of Medicine, Osaka University, Osaka 
      565-0871, Japan. ymori@micro.med.osaka-u.ac.jp
FAU - Akkapaiboon, Pilailuk
AU  - Akkapaiboon P
FAU - Yonemoto, Sayoko
AU  - Yonemoto S
FAU - Koike, Masato
AU  - Koike M
FAU - Takemoto, Masaya
AU  - Takemoto M
FAU - Sadaoka, Tomohiko
AU  - Sadaoka T
FAU - Sasamoto, Yumi
AU  - Sasamoto Y
FAU - Konishi, Shozo
AU  - Konishi S
FAU - Uchiyama, Yasuo
AU  - Uchiyama Y
FAU - Yamanishi, Koichi
AU  - Yamanishi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, CD)
RN  - 0 (CD46 protein, human)
RN  - 0 (Ligands)
RN  - 0 (Membrane Cofactor Protein)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein L, Human herpesvirus 1)
RN  - 0 (glycoprotein O, cytomegalovirus)
RN  - 142985-75-9 (glycoprotein H, herpesvirus 6)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, CD/*metabolism
MH  - Cell Line
MH  - Herpesvirus 6, Human/*metabolism
MH  - Humans
MH  - Ligands
MH  - Membrane Cofactor Protein
MH  - Membrane Glycoproteins/*metabolism
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - T-Lymphocytes/virology
MH  - Viral Envelope Proteins/chemistry/genetics/*metabolism
PMC - PMC387711
EDAT- 2004/04/14 05:00
MHDA- 2004/06/02 05:00
PMCR- 2004/05/01
CRDT- 2004/04/14 05:00
PHST- 2004/04/14 05:00 [pubmed]
PHST- 2004/06/02 05:00 [medline]
PHST- 2004/04/14 05:00 [entrez]
PHST- 2004/05/01 00:00 [pmc-release]
AID - 2279 [pii]
AID - 10.1128/jvi.78.9.4609-4616.2004 [doi]
PST - ppublish
SO  - J Virol. 2004 May;78(9):4609-16. doi: 10.1128/jvi.78.9.4609-4616.2004.