PMID- 15079821
OWN - NLM
STAT- MEDLINE
DCOM- 20041220
LR  - 20061115
IS  - 1099-498X (Print)
IS  - 1099-498X (Linking)
VI  - 6
IP  - 4
DP  - 2004 Apr
TI  - RNA-containing adenovirus/polyethylenimine transfer complexes effectively 
      transduce dendritic cells and induce antigen-specific T cell responses.
PG  - 464-70
AB  - BACKGROUND: Dendritic cells (DCs) are the most potent antigen-presenting cells in 
      initiating primary immune responses. Given the unique properties of DCs, 
      gene-modified DCs represent a particularly attractive approach for immunotherapy 
      of diseases such as cancer. METHODS: Gene-modified DCs were obtained by a 
      receptor-mediated gene delivery system using adenovirus (Ad) particles as ligand 
      and RNA or DNA condensed by polyethylenimine (PEI). In vitro transcribed 
      polyadenylated or non-polyadenylated RNA was used. RNA-transduced DCs were 
      generated expressing chicken ovalbumin (OVA) or chimeric constructs thereof, and 
      compared with DNA-transduced DCs. RESULTS: Ad/PEI transfection complexes 
      efficiently delivered RNA into DCs. Such RNA-transduced DCs induced OVA-specific 
      T cell responses more effectively than DNA-transduced DCs. Furthermore, DCs 
      transduced with polyadenylated RNA were more potent in stimulating CD4(+) and 
      CD8(+) T cell responses than DCs transduced with non-polyadenylated RNA and this 
      was particularly important for CD4(+) T cell responses. CONCLUSIONS: Ad/PEI/RNA 
      transfection is an efficient means for generating RNA-transduced DCs and for 
      stimulating antigen-specific T cell responses. Polyadenylation of RNA enhances 
      CD8(+) T cell responses and is essential for CD4(+) T cell responses.
CI  - Copyright 2004 John Wiley & Sons, Ltd.
FAU - Gust, Tatjana C
AU  - Gust TC
AD  - Max-Delbruck-Center for Molecular Medicine, MDC, Robert-Rossle-Str. 10, D-13092 
      Berlin, Germany.
FAU - Diebold, Sandra S
AU  - Diebold SS
FAU - Cotten, Matt
AU  - Cotten M
FAU - Zenke, Martin
AU  - Zenke M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gene Med
JT  - The journal of gene medicine
JID - 9815764
RN  - 0 (Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Macromolecular Substances)
RN  - 63231-63-0 (RNA)
RN  - 9002-98-6 (Polyethyleneimine)
RN  - 9006-59-1 (Ovalbumin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adenoviridae/*genetics
MH  - Animals
MH  - Antigens/genetics
MH  - CD4-Positive T-Lymphocytes/physiology
MH  - CD8-Positive T-Lymphocytes/physiology
MH  - Cells, Cultured
MH  - DNA/administration & dosage
MH  - Dendritic Cells/*physiology
MH  - Histocompatibility Antigens Class II/genetics/immunology
MH  - Lymphocyte Activation/genetics
MH  - Macromolecular Substances
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Ovalbumin/genetics
MH  - Polyadenylation
MH  - Polyethyleneimine
MH  - RNA/*administration & dosage/chemistry
MH  - T-Lymphocytes/*immunology/*physiology
MH  - Transduction, Genetic/*methods
MH  - Transfection/methods
EDAT- 2004/04/14 05:00
MHDA- 2004/12/21 09:00
CRDT- 2004/04/14 05:00
PHST- 2004/04/14 05:00 [pubmed]
PHST- 2004/12/21 09:00 [medline]
PHST- 2004/04/14 05:00 [entrez]
AID - 10.1002/jgm.492 [doi]
PST - ppublish
SO  - J Gene Med. 2004 Apr;6(4):464-70. doi: 10.1002/jgm.492.