PMID- 15080835
OWN - NLM
STAT- MEDLINE
DCOM- 20040809
LR  - 20051117
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 59
IP  - 5
DP  - 2004 May
TI  - Molecular characterization of a 10-kDa buckwheat molecule reactive to allergic 
      patients' IgE.
PG  - 533-8
AB  - BACKGROUND: Using the sera from buckwheat (BW)-allergic patients, several 
      putative causative molecules were reported. However, few molecules were 
      determined on the molecular structure. We demonstrated in 2000 that the major 
      allergen with 24 kDa (BW24KD) is a legumin-like storage protein. OBJECTIVE: The 
      aim of this study was to isolate and characterize further a major allergen with 
      10 kDa by molecular cloning. METHODS AND RESULTS: Buckwheat allergens were 
      identified by immunoblotting analysis using sera from 14 allergic and two 
      nonallergic individuals. We identified a protein with 10 kDa (BW10KD) that 
      reacted with immunoglobulin E (IgE) more strongly than with IgG and IgA in 57% of 
      the allergic patients but not with IgE in nonallergic individuals. Analyses were 
      performed by N-terminal amino acid sequencing and molecular cloning. 
      Physiological significance was assessed by an immunoblotting experiment showing 
      that the reactivity of an allergic patient's serum IgE to BW10KD was 
      competitively inhibited by natural BW extracts. CONCLUSION: Molecular cloning 
      experiments indicated that BW10KD as a BW allergen was a member of the 2S-albumin 
      multigene family.
FAU - Matsumoto, R
AU  - Matsumoto R
AD  - Department of Bioengineering, Faculty of Engineering, Kagoshima University, 
      Kagoshima, Japan.
FAU - Fujino, K
AU  - Fujino K
FAU - Nagata, Y
AU  - Nagata Y
FAU - Hashiguchi, S
AU  - Hashiguchi S
FAU - Ito, Y
AU  - Ito Y
FAU - Aihara, Y
AU  - Aihara Y
FAU - Takahashi, Y
AU  - Takahashi Y
FAU - Maeda, K
AU  - Maeda K
FAU - Sugimura, K
AU  - Sugimura K
LA  - eng
SI  - GENBANK/AB055892
PT  - Journal Article
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Allergens)
RN  - 0 (Plant Extracts)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Allergens/chemistry/*genetics/*immunology/isolation & purification
MH  - Amino Acid Sequence
MH  - Child
MH  - Child, Preschool
MH  - Fagopyrum/chemistry/*immunology
MH  - Female
MH  - Humans
MH  - Hypersensitivity/*immunology
MH  - Immunoblotting
MH  - Immunoglobulin E/*immunology
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Plant Extracts/chemistry
EDAT- 2004/04/15 05:00
MHDA- 2004/08/10 05:00
CRDT- 2004/04/15 05:00
PHST- 2004/04/15 05:00 [pubmed]
PHST- 2004/08/10 05:00 [medline]
PHST- 2004/04/15 05:00 [entrez]
AID - ALL412 [pii]
AID - 10.1046/j.1398-9995.2003.00412.x [doi]
PST - ppublish
SO  - Allergy. 2004 May;59(5):533-8. doi: 10.1046/j.1398-9995.2003.00412.x.