PMID- 15080837
OWN - NLM
STAT- MEDLINE
DCOM- 20040809
LR  - 20131121
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 59
IP  - 5
DP  - 2004 May
TI  - Neuronal nitric oxide synthase gene polymorphism and IgE-mediated allergy in the 
      Central European population.
PG  - 548-52
AB  - BACKGROUND: Several findings suggest that nitric oxide (NO) plays a significant 
      role in the regulation of the Th1/Th2 balance and contributes to the development 
      of allergic diseases. Our study investigates a possible association of C/T 
      transition located 276-bp downstream from the translation termination site in 
      exon 29 of the human nitric oxide synthase type 1 (NOS1) gene with immunoglobulin 
      E (IgE)-mediated allergic diseases in the Czech population. METHODS: The study 
      included 688 subjects - 368 patients with clinically manifested allergic diseases 
      and 320 unrelated controls with negative familial history of asthma/atopy. The 
      NOS1 genotypes were determined by polymerase chain reaction (PCR) and restriction 
      analysis by Eco72I. RESULTS: No significant differences were found for allele or 
      genotype frequencies of the 5266 C/T polymorphism in exon 29 of the NOS1 gene 
      between IgE-mediated allergic diseases (or asthma alone) and healthy subjects. 
      However, this common polymorphism showed a significant association with signs of 
      atopy, especially with total serum IgE levels [log(e) IgE levels (mean +/- SD): 
      CC genotype = 4.34 +/- 1.40; CT genotype = 4.58 +/- 1.53; TT genotype = 5.01 +/- 
      1.61; P < 0.05). CONCLUSIONS: Our findings suggest that NOS1 gene may participate 
      in the pathogenesis of high total serum IgE levels in allergic diseases in our 
      population. These findings provide support for NOS1 as a candidate gene for 
      IgE-mediated allergy.
FAU - Holla, L I
AU  - Holla LI
AD  - Department of Pathological Physiology, Masaryk University Brno, Brno, Czech 
      Republic.
FAU - Schuller, M
AU  - Schuller M
FAU - Buckova, D
AU  - Buckova D
FAU - Vacha, J
AU  - Vacha J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 8J337D1HZY (Cytosine)
RN  - EC 1.14.13.39 (NOS1 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
RN  - QR26YLT7LT (Thymine)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Alleles
MH  - Case-Control Studies
MH  - Cytosine
MH  - Czech Republic
MH  - Female
MH  - Gene Frequency
MH  - Genotype
MH  - Humans
MH  - Hypersensitivity/blood/*genetics/*immunology
MH  - Immunoglobulin E/blood/*immunology
MH  - Male
MH  - Nitric Oxide Synthase/*genetics
MH  - Nitric Oxide Synthase Type I
MH  - *Polymorphism, Genetic
MH  - Thymine
EDAT- 2004/04/15 05:00
MHDA- 2004/08/10 05:00
CRDT- 2004/04/15 05:00
PHST- 2004/04/15 05:00 [pubmed]
PHST- 2004/08/10 05:00 [medline]
PHST- 2004/04/15 05:00 [entrez]
AID - ALL458 [pii]
AID - 10.1111/j.1398-9995.2004.00458.x [doi]
PST - ppublish
SO  - Allergy. 2004 May;59(5):548-52. doi: 10.1111/j.1398-9995.2004.00458.x.