PMID- 15084406
OWN - NLM
STAT- MEDLINE
DCOM- 20040903
LR  - 20040415
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 102
IP  - 2
DP  - 2004 Jun 15
TI  - GP5 ectodomain epitope of porcine reproductive and respiratory syndrome virus, 
      strain Lelystad virus.
PG  - 225-30
AB  - Sera from pigs infected with the European porcine reproductive and respiratory 
      syndrome virus (PRRSV), strain Lelystad virus (LV), were screened by indirect 
      ELISA for antibodies that bound to a series of overlapping synthetic peptides 
      covering amino acids (AA) 19-60 of the primary envelope glycoprotein (GP)5. 
      Antibodies were detected that bound to an epitope(s) located in an ectodomain 
      segment composed of AA 38-54. The antibodies strongly cross-reacted with peptides 
      specific for the North American PRRSV VR2332. Antibodies with the same 
      specificity were present in sera of pigs infected in the US with a European-like 
      PRRSV. Competitive ELISA using overlapping 8-10-AA-long peptides confirmed that 
      the epitope recognized by the antibodies from LV-infected pigs is located in the 
      same segment as the neutralization epitopes previously identified for PRRSV 
      VR2332 and the closely related arterivirus, lactate dehydrogenase-elevating virus 
      (LDV). No antibodies were detected that bound to synthetic peptides representing 
      further upstream GP5 segments that have been reported to carry neutralization or 
      non-neutralization epitopes of some PRRSV isolates.
FAU - Plagemann, Peter G W
AU  - Plagemann PG
AD  - Department of Microbiology, University of Minnesota, MMC 196, Minneapolis, MN 
      55455, USA. plage001@umn.edu
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Peptide Library)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood/immunology
MH  - Antigens, Viral/*immunology
MH  - Cross Reactions
MH  - Epitope Mapping
MH  - Epitopes/*immunology
MH  - Neutralization Tests
MH  - Peptide Library
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - Viral Envelope Proteins/*immunology
EDAT- 2004/04/16 05:00
MHDA- 2004/09/04 05:00
CRDT- 2004/04/16 05:00
PHST- 2003/11/20 00:00 [received]
PHST- 2004/01/26 00:00 [revised]
PHST- 2004/01/26 00:00 [accepted]
PHST- 2004/04/16 05:00 [pubmed]
PHST- 2004/09/04 05:00 [medline]
PHST- 2004/04/16 05:00 [entrez]
AID - S0168170204000723 [pii]
AID - 10.1016/j.virusres.2004.01.031 [doi]
PST - ppublish
SO  - Virus Res. 2004 Jun 15;102(2):225-30. doi: 10.1016/j.virusres.2004.01.031.