PMID- 15085304
OWN - NLM
STAT- MEDLINE
DCOM- 20041109
LR  - 20181113
IS  - 0095-3628 (Print)
IS  - 0095-3628 (Linking)
VI  - 47
IP  - 3
DP  - 2004 Apr
TI  - FISH shows that Desulfotomaculum spp. are the dominating sulfate-reducing 
      bacteria in a pristine aquifer.
PG  - 236-42
AB  - The hydrochemistry and the microbial diversity of a pristine aquifer system near 
      Garzweiler, Germany, were characterized. Hydrogeochemical and isotopic data 
      indicate a recent activity of sulfate-reducing bacteria in the Tertiary marine 
      sands. The community structure in the aquifer was studied by fluorescence in situ 
      hybridization (FISH). Up to 7.3 x 10(5) cells/mL were detected by DAPI-staining. 
      Bacteria (identified by the probe EUB338) were dominant, representing 51.9% of 
      the total cell number (DAPI). Another 25.7% of total cell were affiliated with 
      the domain Archaea as identified by the probe ARCH915. Within the domain 
      Bacteria, the beta-Proteobacteria were most abundant (21.0% of total cell 
      counts). Using genus-specific probes for sulfate-reducing bacteria (SRB), 2.5% of 
      the total cells were identified as members of the genus Desulfotomaculum. This 
      reflects the predominant role these microorganisms have been found to play in 
      sulfate-reducing zones of aquifers at other sites. Previously, all SRB cultured 
      from this site were from the spore-forming genera Desulfotomaculum and 
      Desulfosporosinus.
FAU - Detmers, J
AU  - Detmers J
AD  - Department of Microbiology, Max-Planck-Institute for Marine Microbiology, 
      Celsiusstrasse 1, D-28359 Bremen, Germany.
FAU - Strauss, H
AU  - Strauss H
FAU - Schulte, U
AU  - Schulte U
FAU - Bergmann, A
AU  - Bergmann A
FAU - Knittel, K
AU  - Knittel K
FAU - Kuever, J
AU  - Kuever J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031023
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
RN  - 0 (Oligonucleotides)
SB  - IM
MH  - Archaea/genetics/physiology
MH  - Desulfotomaculum/genetics/*physiology
MH  - *Ecosystem
MH  - Fresh Water/chemistry/*microbiology
MH  - Germany
MH  - In Situ Hybridization, Fluorescence
MH  - Likelihood Functions
MH  - Models, Genetic
MH  - Oligonucleotides
MH  - *Phylogeny
MH  - Sequence Analysis, DNA
MH  - *Water Microbiology
EDAT- 2004/04/16 05:00
MHDA- 2004/11/13 09:00
CRDT- 2004/04/16 05:00
PHST- 2003/02/19 00:00 [received]
PHST- 2003/07/10 00:00 [accepted]
PHST- 2004/04/16 05:00 [pubmed]
PHST- 2004/11/13 09:00 [medline]
PHST- 2004/04/16 05:00 [entrez]
AID - 10.1007/s00248-004-9952-6 [doi]
PST - ppublish
SO  - Microb Ecol. 2004 Apr;47(3):236-42. doi: 10.1007/s00248-004-9952-6. Epub 2003 Oct 
      23.