PMID- 15085752
OWN - NLM
STAT- MEDLINE
DCOM- 20040519
LR  - 20180217
IS  - 0001-5547 (Print)
IS  - 0001-5547 (Linking)
VI  - 48
IP  - 2
DP  - 2004 Mar-Apr
TI  - Overexpression of HER2/neu oncoprotein in cytologic specimens.
PG  - 199-206
AB  - OBJECTIVE: To assess the rate of HER2/neu overexpression in cytologic specimens 
      by immunocytochemistry (ICC) and compare these results in matched surgical 
      specimens by immunohistochemistry (IHC) and fluorescence in situ hybridization 
      (FISH), when available. STUDY DESIGN: All cytologic specimens processed for 
      HER2/neu evaluation by ICC (72 cases) and available corresponding histologic 
      specimens (16 cases) were retrieved from our files. ICC was applied to previously 
      Papanicolaou stained, routine fine needle aspirations specimens (64 cases) and 
      cytocentrifuged, alcohol-fixed, fluid specimens (8 cases). FISH was performed on 
      6 histologic specimens. RESULTS: Overexpression of HER2/neu was seen in 7/22 
      breast cancers (31.8%), 3/18 pulmonary adenocarcinomas (16.6%), 2/5 colorectal 
      adenocarcinomas (40%), 1/2 adenocarcinomas of the biliary system (50%), 1/3 
      thyroid papillary carcinomas (33.3%) and 1/3 prostate adenocarcinomas (33.3%). 
      Sixteen cases had IHC in matched histologic specimens: 14 (87.5%) cases were 
      concordant (11 negative and 3 positive in both specimens), 1 case was negative in 
      the cytologic specimen and positive in the histologic specimen (with no 
      amplification by FISH), and 1 case was positive in the cytologic specimen and 
      negative in the histologic specimen (not informative by FISH). CONCLUSION: Our 
      data suggest that overexpression of HER2/neu oncoprotein can be successfully 
      detected in routine cytologic specimens, providing a simple, fast and 
      cost-effective method of selecting patients for specific treatment.
FAU - Mitteldorf, Cristina A
AU  - Mitteldorf CA
AD  - Laboratory of Surgical and Molecular Pathology, Hospital Sirio Libanes, Sao 
      Paulo, SP, Brazil. crismitteldorf@ig.com.br
FAU - Leite, Katia R
AU  - Leite KR
FAU - Meirelles, Maria Ines
AU  - Meirelles MI
FAU - Gattas, Gilka J
AU  - Gattas GJ
FAU - Camara-Lopes, L H
AU  - Camara-Lopes LH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Switzerland
TA  - Acta Cytol
JT  - Acta cytologica
JID - 0370307
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adenocarcinoma/metabolism/pathology
MH  - Biomarkers, Tumor/analysis/*biosynthesis
MH  - Carcinoma, Papillary/metabolism/pathology
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Neoplasms/*metabolism/*pathology
MH  - Predictive Value of Tests
MH  - Receptor, ErbB-2/analysis/*biosynthesis
MH  - Reproducibility of Results
MH  - Up-Regulation/physiology
EDAT- 2004/04/17 05:00
MHDA- 2004/05/20 05:00
CRDT- 2004/04/17 05:00
PHST- 2004/04/17 05:00 [pubmed]
PHST- 2004/05/20 05:00 [medline]
PHST- 2004/04/17 05:00 [entrez]
AID - 10.1159/000326316 [doi]
PST - ppublish
SO  - Acta Cytol. 2004 Mar-Apr;48(2):199-206. doi: 10.1159/000326316.