PMID- 15087548
OWN - NLM
STAT- MEDLINE
DCOM- 20040428
LR  - 20131121
IS  - 1095-9203 (Electronic)
IS  - 0036-8075 (Linking)
VI  - 304
IP  - 5669
DP  - 2004 Apr 16
TI  - Functional stoichiometry and local enrichment of calmodulin interacting with Ca2+ 
      channels.
PG  - 432-5
AB  - Calmodulin (CaM) interactions with Ca2+ channels mediate both Ca2+ regulation of 
      channels and local Ca2+ triggering of transcription factors implicated in 
      neuronal memory. Crucial to these functions are the number of CaM molecules 
      (CaMs) regulating each channel, and the number of CaMs privy to the local Ca2+ 
      signal from each channel. To resolve these parameters, we fused L-type Ca2+ 
      channels to single CaM molecules. These chimeric molecules revealed that a single 
      CaM directs L-type channel regulation. Similar fusion molecules were used to 
      estimate the local CaM concentration near Ca2+ channels. This estimate indicates 
      marked enrichment of local CaM, as if a "school" of nearby CaMs were poised to 
      enhance the transduction of local Ca2+ entry into diverse signaling pathways.
FAU - Mori, Masayuki X
AU  - Mori MX
AD  - Ca2+ Signals Laboratory, Department of Biomedical Engineering , Johns Hopkins 
      University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA.
FAU - Erickson, Michael G
AU  - Erickson MG
FAU - Yue, David T
AU  - Yue DT
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Calcium Channels, L-Type)
RN  - 0 (Calmodulin)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (L-type calcium channel alpha(1C))
RN  - 0 (Peptides)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 25718-94-9 (polyglycine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
CIN - Science. 2004 Apr 16;304(5669):394-5. PMID: 15087533
MH  - Calcium/*metabolism
MH  - Calcium Channels, L-Type/chemistry/*metabolism
MH  - Calcium Signaling
MH  - Calmodulin/chemistry/genetics/*metabolism
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Fluorescence Resonance Energy Transfer
MH  - Humans
MH  - Mathematics
MH  - Mutation
MH  - Patch-Clamp Techniques
MH  - Peptides/chemistry/genetics
MH  - Protein Structure, Tertiary
MH  - Recombinant Fusion Proteins/chemistry/metabolism
MH  - Transfection
EDAT- 2004/04/17 05:00
MHDA- 2004/04/29 05:00
CRDT- 2004/04/17 05:00
PHST- 2004/04/17 05:00 [pubmed]
PHST- 2004/04/29 05:00 [medline]
PHST- 2004/04/17 05:00 [entrez]
AID - 304/5669/432 [pii]
AID - 10.1126/science.1093490 [doi]
PST - ppublish
SO  - Science. 2004 Apr 16;304(5669):432-5. doi: 10.1126/science.1093490.