PMID- 15093279
OWN - NLM
STAT- MEDLINE
DCOM- 20040524
LR  - 20161124
IS  - 0378-4274 (Print)
IS  - 0378-4274 (Linking)
VI  - 149
IP  - 1-3
DP  - 2004 Apr 1
TI  - Cytogenetic analysis using fluorescence in situ hybridization (FISH) to evaluate 
      occupational exposure to carcinogens.
PG  - 335-44
AB  - Chromosomal aberrations determined by conventional method or fluorescence in situ 
      hybridization (FISH) technique with whole chromosome painting are used as 
      biomarkers of effect. Groups occupationally exposed to 1,3-butadiene (BD), 
      acrylonitrile, ethyl benzene and benzene in petrochemical industry, and 
      carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) from ambient air were 
      followed by conventional method and FISH painting for chromosomes # 1 and # 4, in 
      total 383 subjects, including controls. No effect was observed by either method 
      with exposure to 1,3-butadiene < 1mg/m(3) and acrylonitrile < 0.3mg/m(3). Ethyl 
      benzene and benzene exposure significantly increased chromosomal aberrations by 
      both methods, which decreased after the implementation of preventive measures. 
      The genomic frequency of translocations by FISH calculated as FG/100 was 
      significantly increased in city policemen versus control group exposed to c-PAHs 
      from ambient air (1.72+/-1.57 versus 1.25+/-1.11, P<0.05). The method of FISH 
      with whole chromosome painting seems to be more sensitive to detect chromosomal 
      injury by occupational exposure to carcinogens than conventional method.
FAU - Sram, Radim J
AU  - Sram RJ
AD  - Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR and 
      Health Institute of Central Bohemia, 142 20 Prague 4, Czech Republic. 
      sram@biomed.cas.cz
FAU - Beskid, Olena
AU  - Beskid O
FAU - Binkova, Blanka
AU  - Binkova B
FAU - Rossner, Pavel
AU  - Rossner P
FAU - Smerhovsky, Zdenek
AU  - Smerhovsky Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Benzene Derivatives)
RN  - 0 (Butadienes)
RN  - 0 (Carcinogens)
RN  - 0 (Genetic Markers)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - JSD5FGP5VD (1,3-butadiene)
RN  - L5I45M5G0O (ethylbenzene)
RN  - MP1U0D42PE (Acrylonitrile)
SB  - IM
MH  - Acrylonitrile/analysis/toxicity
MH  - Adult
MH  - Benzene Derivatives/analysis/toxicity
MH  - Butadienes/analysis/toxicity
MH  - Carcinogens/*analysis
MH  - Chemical Industry
MH  - Chromosome Aberrations/chemically induced
MH  - Chromosome Painting
MH  - *Cytogenetic Analysis
MH  - Female
MH  - Genetic Markers
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Occupational Exposure/*adverse effects
MH  - Police
MH  - Polycyclic Aromatic Hydrocarbons/analysis/toxicity
EDAT- 2004/04/20 05:00
MHDA- 2004/05/25 05:00
CRDT- 2004/04/20 05:00
PHST- 2004/04/20 05:00 [pubmed]
PHST- 2004/05/25 05:00 [medline]
PHST- 2004/04/20 05:00 [entrez]
AID - S0378427403005125 [pii]
AID - 10.1016/j.toxlet.2003.12.043 [doi]
PST - ppublish
SO  - Toxicol Lett. 2004 Apr 1;149(1-3):335-44. doi: 10.1016/j.toxlet.2003.12.043.