PMID- 15094197
OWN - NLM
STAT- MEDLINE
DCOM- 20040806
LR  - 20061115
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 331
DP  - 2004 Apr 28
TI  - Cloning, expression and subcellular localization of HN1 and HN1L genes, as well 
      as characterization of their orthologs, defining an evolutionarily conserved gene 
      family.
PG  - 115-23
AB  - The present work reported the cloning and characterization of two novel human 
      genes--HN1 (hematopoietic- and neurologic-expressed sequence 1) and HN1L 
      (HN1-like gene) which are proposed to be involved in embryo development. HN1 is 
      mapped on chromosome 17q25.2, with two transcripts (1.0 and 1.6 kb in length, 
      respectively) due to alternative splicing. HN1 is expressed abundantly in testis 
      and skeletal muscle among 16 human tissues, and it is localized in the nucleus 
      indicated by GFP fusion expression. Western blot confirmed that HN1 encodes a 
      16.5-kDa protein. HN1L is on chromosome 16p13.3, with three splicing in the 
      length of 2.0, 4.0 and 4.2 kb, respectively. HN1L is expressed in a variety of 
      tissues such as liver, kidney, prostate, testis and uterus at varying levels. 
      HN1L gene encodes a 20-kDa protein, which is localized in both the nucleus and 
      cytoplasm. Fourteen of HN1 and sixteen of HN1L homologous genes in different 
      species were determined and analyzed by BLAST searches. Silicon analyses of the 
      14 orthologous proteins of HN1 and 16 orthologous proteins of HN1L revealed that 
      they share great conservation in vertebrate. Additionally, we identified nine 
      pseudogenes of HN1 (six) and HN1L (three) in the genomes of the human, mouse and 
      rat. Based on sequence alignments and phylogenetic analysis, all these homologous 
      genes and pseudogenes were defined as a HN1 gene family.
FAU - Zhou, Guangjin
AU  - Zhou G
AD  - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of 
      Life Science, Fudan University, 220 Handan Road, Shanghai 200433, PR China.
FAU - Wang, Jian
AU  - Wang J
FAU - Zhang, Yue
AU  - Zhang Y
FAU - Zhong, Chaomin
AU  - Zhong C
FAU - Ni, Jun
AU  - Ni J
FAU - Wang, Li
AU  - Wang L
FAU - Guo, Jinhu
AU  - Guo J
FAU - Zhang, Kexiong
AU  - Zhang K
FAU - Yu, Long
AU  - Yu L
FAU - Zhao, Shouyuan
AU  - Zhao S
LA  - eng
SI  - GENBANK/AF086910
SI  - GENBANK/AY323831
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Complementary)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Motifs/genetics
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Conserved Sequence/genetics
MH  - DNA/chemistry/genetics
MH  - DNA, Complementary/chemistry/genetics
MH  - *Evolution, Molecular
MH  - Exons
MH  - Female
MH  - *Gene Expression Profiling
MH  - Green Fluorescent Proteins
MH  - HeLa Cells
MH  - Humans
MH  - Introns
MH  - Luminescent Proteins/genetics/metabolism
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/*genetics/metabolism
MH  - Phylogeny
MH  - Pseudogenes/genetics
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Transfection
EDAT- 2004/04/20 05:00
MHDA- 2004/08/07 05:00
CRDT- 2004/04/20 05:00
PHST- 2003/09/23 00:00 [received]
PHST- 2004/01/20 00:00 [revised]
PHST- 2004/02/04 00:00 [accepted]
PHST- 2004/04/20 05:00 [pubmed]
PHST- 2004/08/07 05:00 [medline]
PHST- 2004/04/20 05:00 [entrez]
AID - S0378111904000691 [pii]
AID - 10.1016/j.gene.2004.02.025 [doi]
PST - ppublish
SO  - Gene. 2004 Apr 28;331:115-23. doi: 10.1016/j.gene.2004.02.025.