PMID- 1509507
OWN - NLM
STAT- MEDLINE
DCOM- 19920924
LR  - 20190819
IS  - 0378-4274 (Print)
IS  - 0378-4274 (Linking)
VI  - 62
IP  - 1
DP  - 1992 Aug
TI  - Detection of contact sensitivity of metal salts using the murine local lymph node 
      assay.
PG  - 53-61
AB  - The local lymph node assay (LLNA) is a predictive test for the detection of 
      contact allergens. Nickel and chromium sensitization are common cases in man. 
      However, in a previous study topical application of nickel sulfate and potassium 
      dichromate in aqueous solution failed to induce activation in the draining lymph 
      node. This study describes the application of LLNA to evaluate the contact 
      sensitivity of metal salts. The metal salts were applied in dimethylsulfoxide or 
      aqueous ethanol solution. In some experiments, the skin of the ears was gently 
      abraded using a needle prior to application of metal salts. The ability of seven 
      metal salts to induce lymph node cell (LNC) proliferation was compared. Nickel, 
      cobalt, chromium and copper salts increased LNC proliferation, whereas zinc, 
      manganese and iron salts failed to induce LNC proliferation in this assay.
FAU - Ikarashi, Y
AU  - Ikarashi Y
AD  - National Institute of Hygienic Sciences, Tokyo, Japan.
FAU - Tsuchiya, T
AU  - Tsuchiya T
FAU - Nakamura, A
AU  - Nakamura A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Ferrous Compounds)
RN  - 0 (Metals)
RN  - 0 (Pharmaceutical Vehicles)
RN  - 0 (Sulfates)
RN  - 39R4TAN1VT (ferrous sulfate)
RN  - 3G0H8C9362 (Cobalt)
RN  - 3K9958V90M (Ethanol)
RN  - 7733-02-0 (Zinc Sulfate)
RN  - EVS87XF13W (cobaltous chloride)
RN  - J41CSQ7QDS (Zinc)
RN  - T4423S18FM (Potassium Dichromate)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
MH  - Animals
MH  - Cell Division/drug effects
MH  - Cobalt/toxicity
MH  - Dermatitis, Contact/*etiology
MH  - Dimethyl Sulfoxide/pharmacology
MH  - Ear, External
MH  - Ethanol/pharmacology
MH  - Female
MH  - Ferrous Compounds/toxicity
MH  - Lymph Nodes/cytology/*drug effects
MH  - Lymphocyte Activation/drug effects
MH  - Metals/*toxicity
MH  - Mice
MH  - Pharmaceutical Vehicles/pharmacology
MH  - Potassium Dichromate/toxicity
MH  - Skin/drug effects
MH  - Skin Tests/methods
MH  - Sulfates/toxicity
MH  - Zinc/toxicity
MH  - Zinc Sulfate
EDAT- 1992/08/01 00:00
MHDA- 1992/08/01 00:01
CRDT- 1992/08/01 00:00
PHST- 1992/08/01 00:00 [pubmed]
PHST- 1992/08/01 00:01 [medline]
PHST- 1992/08/01 00:00 [entrez]
AID - 0378-4274(92)90078-X [pii]
AID - 10.1016/0378-4274(92)90078-x [doi]
PST - ppublish
SO  - Toxicol Lett. 1992 Aug;62(1):53-61. doi: 10.1016/0378-4274(92)90078-x.