PMID- 15095455 OWN - NLM STAT- MEDLINE DCOM- 20050224 LR - 20061115 IS - 0173-0835 (Print) IS - 0173-0835 (Linking) VI - 25 IP - 7-8 DP - 2004 Apr TI - Electroosmotic flow in a poly(dimethylsiloxane) channel does not depend on percent curing agent. PG - 1120-4 AB - Poly(dimethylsiloxane) (PDMS) microfluidic devices were prepared from different ratios of "curing agent" (which contains silicon hydride groups) to "base" (which contains vinyl-terminated noncross-linked PDMS), to determine the effect of this ratio on electroosmotic flow (EOF). In fabricating devices for this purpose, a novel method for permanently enclosing PDMS channels was developed. As a supplement to the microfluidic method, the inner walls of capillaries were coated with PDMS formed from varying ratios of curing agent to base. EOF was found to be constant for PDMS formed with each ratio, which implies that the negative surface charges do not arise from chemical species present only in the base or the curing agent. FAU - Wheeler, Aaron R AU - Wheeler AR AD - Department of Chemistry, Stanford University, Stanford, CA 94305-5080, USA. FAU - Trapp, Gabriele AU - Trapp G FAU - Trapp, Oliver AU - Trapp O FAU - Zare, Richard N AU - Zare RN LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Germany TA - Electrophoresis JT - Electrophoresis JID - 8204476 RN - 0 (Dimethylpolysiloxanes) RN - 0 (Silicones) RN - 63148-62-9 (baysilon) SB - IM MH - Dimethylpolysiloxanes/*chemistry MH - Electrophoresis, Capillary/*instrumentation MH - Osmosis MH - Silicones/*chemistry MH - Spectrophotometry, Infrared EDAT- 2004/04/20 05:00 MHDA- 2005/02/25 09:00 CRDT- 2004/04/20 05:00 PHST- 2004/04/20 05:00 [pubmed] PHST- 2005/02/25 09:00 [medline] PHST- 2004/04/20 05:00 [entrez] AID - 10.1002/elps.200305784 [doi] PST - ppublish SO - Electrophoresis. 2004 Apr;25(7-8):1120-4. doi: 10.1002/elps.200305784.