PMID- 15096186
OWN - NLM
STAT- MEDLINE
DCOM- 20040621
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 112
IP  - 1
DP  - 2004 May
TI  - Adoptive transfer of dendritic cells from allergic mice induces specific 
      immunoglobulin E antibody in naive recipients in absence of antigen challenge 
      without altering the T helper 1/T helper 2 balance.
PG  - 72-9
AB  - Dendritic cells (DCs) are important in the regulation of immune responses and it 
      has been proposed that these cells play an important role in asthma; however, 
      their role in food allergy is still largely unknown. Our aim was to study 
      specific immunoglobulin E (IgE) and immunoglobulin G (IgG) responses in naive 
      recipients following adoptive transfer of myeloid DCs from allergic and control 
      mice. The phenotypic features and lymphokine production of DCs were also 
      investigated. CD11c+/hi B220- DCs isolated from spleen and Peyer's patches (PP) 
      of cow's milk (CM) allergic and control mice were transferred intravenously 
      (i.v.) into naive syngeneic recipients, and IgE- and IgG-specific responses were 
      evaluated. Experiments were also carried out to determine the levels of 
      interferon-gamma (IFN-gamma) and interleukin (IL)-4 produced by splenocytes from 
      naive recipients following the adoptive transfer, and CD40 ligand 
      (CD40L)-mediated IL-10 production by DCs from allergic and control mice. DCs 
      isolated from spleen and PP of allergic mice, but not control groups, induced 
      CM-specific IgG and IgE antibody production in naive recipients in the absence of 
      previous immunization, but did not modify the T helper 1 (Th1) and T helper 2 
      (Th2) balance. Furthermore, although no difference was observed in the expression 
      of canonical DC surface markers, PP DCs from allergic mice produced less IL-10 
      than DCs from controls. We interpret these data as showing that DCs play a 
      pivotal role in allergen-specific IgE responses and that a Th2-skewed response 
      may not be involved in the early phase of allergic responses. The identification 
      of the mechanisms underlying these events may help to design novel strategies of 
      therapeutic intervention in food allergy.
FAU - Chambers, Stephen J
AU  - Chambers SJ
AD  - Laboratory of Gut Immunology, Programme of Gastrointestinal Health and Function, 
      Institute of Food Research, Norwich, UK.
FAU - Bertelli, Eugenio
AU  - Bertelli E
FAU - Winterbone, Mark S
AU  - Winterbone MS
FAU - Regoli, Mari
AU  - Regoli M
FAU - Man, Angela L
AU  - Man AL
FAU - Nicoletti, Claudio
AU  - Nicoletti C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (CD40 Antigens)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - CD40 Antigens/immunology
MH  - Dendritic Cells/*immunology/transplantation
MH  - Female
MH  - Immunoglobulin E/*biosynthesis
MH  - Immunophenotyping
MH  - Interleukin-10/biosynthesis
MH  - Interleukin-12/biosynthesis
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Milk Hypersensitivity/*immunology
MH  - Peyer's Patches/immunology
MH  - Spleen/immunology
MH  - Th1 Cells/*immunology
MH  - Th2 Cells/*immunology
PMC - PMC1782460
EDAT- 2004/04/21 05:00
MHDA- 2004/06/23 05:00
PMCR- 2005/05/01
CRDT- 2004/04/21 05:00
PHST- 2004/04/21 05:00 [pubmed]
PHST- 2004/06/23 05:00 [medline]
PHST- 2004/04/21 05:00 [entrez]
PHST- 2005/05/01 00:00 [pmc-release]
AID - IMM1846 [pii]
AID - 10.1111/j.1365-2567.2004.01846.x [doi]
PST - ppublish
SO  - Immunology. 2004 May;112(1):72-9. doi: 10.1111/j.1365-2567.2004.01846.x.