PMID- 15098257
OWN - NLM
STAT- MEDLINE
DCOM- 20040430
LR  - 20191210
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 102
IP  - 2
DP  - 2004 Apr 25
TI  - Rapid detection of the t(11;14) translocation in mantle cell lymphoma by 
      interphase fluorescence in situ hybridization on archival cytopathologic 
      material.
PG  - 124-31
AB  - BACKGROUND: The cytomorphologic diagnosis of mantle cell lymphoma (MCL) can be 
      difficult and requires ancillary studies for accurate subclassification. More 
      than 95% of MCLs are known to carry the t(11;14) chromosomal translocation. 
      However, traditional cytogenetic studies on cytologic material can be both 
      difficult technically and time consuming. Interphase fluorescence in situ 
      hybridization (FISH) can be a powerful tool for detecting chromosomal changes in 
      individual tumor cells. The authors evaluated the utility of interphase FISH for 
      the rapid detection of t(11;14) in archival cytologic material. METHODS: The 
      cytopathology data bases at two institutions were searched for patients with well 
      characterized MCL (biopsy, immunophenotyping). Ten patients with MCL (8 
      fine-needle aspiration samples and 2 body cavity fluid samples) were identified. 
      The area of interest on the cytology slides was marked and hybridized with 
      two-color, locus-specific identifier DNA probes. A dual-fusion probe signal was 
      used to detect the juxtaposition of the immunoglobulin heavy-chain (IgH) (14q32) 
      locus with cyclin D1 (CCND1) gene sequences (11q13). Samples with tumor cell 
      nuclei that showed at least one yellow fusion signal in addition one green signal 
      (IgH) and one orange signal (CCND1) were interpreted as positive. Positive and 
      negative controls were used. RESULTS: The t(11;14) translocation was detected by 
      FISH in 10 of 10 patients (100%) with MCL. CONCLUSIONS: The cytomorphology of 
      small-to-intermediate cell lymphomas, including MCL, follicular lymphoma, and 
      marginal zone/mucosa-associated lymphoid tissue lymphoma, can show overlapping 
      cytomorphologic features with one another as well as with reactive lymphoid 
      proliferations. In selected samples in which specific classification is not 
      possible or when confirmation is required on a small sample size, molecular 
      analysis and cytogenetics may be helpful in arriving at an unambiguous 
      cytodiagnosis and subclassification. Distinction of MCL from other lymphomas is 
      important, because the clinical course is aggressive, and response to 
      conventional chemotherapy is poor. This study showed that the detection of 
      t(11;14) by FISH can be performed rapidly and easily on archival cytologic 
      material for the molecular diagnosis of MCL.
CI  - Copyright 2004 American Cancer Society.
FAU - Bentz, Joel S
AU  - Bentz JS
AD  - Department of Pathology, University of Utah, Salt Lake City, Utah 84108, USA. 
      bentzj@aruplab.com
FAU - Rowe, Leslie R
AU  - Rowe LR
FAU - Anderson, Scott R
AU  - Anderson SR
FAU - Gupta, Prabodh K
AU  - Gupta PK
FAU - McGrath, Cindy M
AU  - McGrath CM
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biopsy
MH  - Chromosome Banding
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 14
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphoma, Mantle-Cell/*genetics/pathology
MH  - Male
MH  - *Translocation, Genetic
EDAT- 2004/04/21 05:00
MHDA- 2004/05/01 05:00
CRDT- 2004/04/21 05:00
PHST- 2004/04/21 05:00 [pubmed]
PHST- 2004/05/01 05:00 [medline]
PHST- 2004/04/21 05:00 [entrez]
AID - 10.1002/cncr.11934 [doi]
PST - ppublish
SO  - Cancer. 2004 Apr 25;102(2):124-31. doi: 10.1002/cncr.11934.