PMID- 15098740 OWN - NLM STAT- MEDLINE DCOM- 20041124 LR - 20190922 IS - 0016-6707 (Print) IS - 0016-6707 (Linking) VI - 121 IP - 1 DP - 2004 May TI - Characterization of a new HpaI centromeric satellite DNA in Salmo salar. PG - 81-7 AB - A highly repeated HpaI DNA family was revealed in Atlantic salmon (Salmo salar) and analyzed by Southern blotting and fluorescence in situ hybridization (FISH). In this report, we describe the nucleotide sequence, genomic structure and chromosomal localization of this HpaI repeat. This novel satellite appeared tandemly arrayed and located at centromeric areas of three acrocentric chromosome pairs as evidenced by FISH. The sequence was characterized by a high AT content (63%), a short consensus motif (A/T)(G/C)AAA(T/C) similar to other centromeric satellites motifs, and by short AT enriched stretches. The presence of this sequence in other salmonid species was also tested by Southern blot hybridization and used to analyze its evolution within this group. FAU - Vinas, Ana AU - Vinas A AD - Departamento de Genetica, Facultad de Biologia, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain. avinas@lugo.usc.es FAU - Abuin, Maria AU - Abuin M FAU - Pardo, Belen G AU - Pardo BG FAU - Martinez, Paulino AU - Martinez P FAU - Sanchez, Laura AU - Sanchez L LA - eng PT - Journal Article PL - Netherlands TA - Genetica JT - Genetica JID - 0370740 RN - 0 (DNA, Satellite) SB - IM MH - Animals MH - Base Sequence MH - Blotting, Southern MH - *Centromere MH - DNA, Satellite/*genetics MH - In Situ Hybridization, Fluorescence MH - Karyotyping MH - Molecular Sequence Data MH - Repetitive Sequences, Nucleic Acid MH - Salmo salar/*genetics EDAT- 2004/04/22 05:00 MHDA- 2004/12/16 09:00 CRDT- 2004/04/22 05:00 PHST- 2004/04/22 05:00 [pubmed] PHST- 2004/12/16 09:00 [medline] PHST- 2004/04/22 05:00 [entrez] AID - 10.1023/b:gene.0000019927.30049.9c [doi] PST - ppublish SO - Genetica. 2004 May;121(1):81-7. doi: 10.1023/b:gene.0000019927.30049.9c.