PMID- 15098742
OWN - NLM
STAT- MEDLINE
DCOM- 20041124
LR  - 20190922
IS  - 0016-6707 (Print)
IS  - 0016-6707 (Linking)
VI  - 121
IP  - 1
DP  - 2004 May
TI  - Chromosomal mapping and molecular characterization of ribosomal RNA genes in 
      Lebias fasciata (Teleostei, Cyprinodontidae).
PG  - 95-100
AB  - Chromosome location of major (18S, 5.8S and 28S) and 5S ribosomal RNA genes 
      (rDNAs) was examined in Lebias fasciata collected from different Italian 
      blackish-waters, using silver (Ag)- and chromomycin A3 (CMA3)-staining and/or 
      fluorescence in situ hybridization (FISH). Both 18S and 5S rDNA probes for FISH 
      were obtained with polymerase chain reaction-directed cloning from genomic DNA of 
      the examined species. Nucleolar organizer regions (NORs) containing the major 
      rDNAs showed intraspecific polymorphism in number as detected by Ag-and 
      CMA3-staining and FISH with the 18S rDNA probe. On the other hand, 5S rDNA loci 
      constantly occurred on one chromosome pair and co-localized with a pair of the 
      major rDNA loci as evidenced by two-color FISH using the 5S and 18S rDNA probes. 
      Sequential CMA3- and Ag-NOR staining and FISH revealed apparent inactivation of 
      some NORs. The cloned 5S rDNA was found to contain some TATA-like sequences that 
      might play an important role in the regulation of gene expression.
FAU - Tigano, C
AU  - Tigano C
AD  - Dipartimento di Biologia Animale, Universita di Catania, Via Androne 81-95124 
      Catania, Italy.
FAU - Rocco, L
AU  - Rocco L
FAU - Ferrito, V
AU  - Ferrito V
FAU - Costagliola, D
AU  - Costagliola D
FAU - Pappalardo, A M
AU  - Pappalardo AM
FAU - Stingo, V
AU  - Stingo V
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Genetica
JT  - Genetica
JID - 0370740
RN  - DVW027E7NL (Chromomycin A3)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromomycin A3/analysis
MH  - Chromosome Banding
MH  - *Chromosome Mapping
MH  - *Genes, rRNA
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Killifishes/*genetics
MH  - Molecular Sequence Data
MH  - Nucleolus Organizer Region
MH  - Silver Staining
EDAT- 2004/04/22 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/04/22 05:00
PHST- 2004/04/22 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/04/22 05:00 [entrez]
AID - 10.1023/b:gene.0000019931.89458.dc [doi]
PST - ppublish
SO  - Genetica. 2004 May;121(1):95-100. doi: 10.1023/b:gene.0000019931.89458.dc.