PMID- 15099758
OWN - NLM
STAT- MEDLINE
DCOM- 20040617
LR  - 20071114
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 287
IP  - 1-2
DP  - 2004 Apr
TI  - The use of class-I HLA tetramers for the detection of hepatitis C virus 
      NS3-specific CD8(+) T cells in patients with chronic infection.
PG  - 91-9
AB  - BACKGROUND/AIMS: New methods to detect virus-specific T-cell responses have 
      recently been developed. Several human leukocyte antigen (HLA)-peptide tetramers 
      for the detection of hepatitis C virus (HCV)-specific CD8(+) T cells are under 
      evaluation. METHODS: Evaluation of one HLA class I-tetramer (HCVNS3-2) for the 
      detection of HCV NS3-specific CD8(+) T cells in a series of 38 HLA-A2(+) 
      chronically infected patients. RESULTS: Almost half (42%) of the patients had 
      detectable NS3-specific CD8(+) T cells. The frequencies of such cells ranged from 
      0.01% to 0.22% of total CD8(+) T cells. No significant differences in clinical 
      features or mean viral load were detected between patients with or without 
      tetramer + CD8(+) T cells. CONCLUSIONS: The tetramer HCVNS3-2 may be very useful 
      for the study of the HCV-specific CD8(+) immune response. Combination of this 
      reagent with other tetramers based on other HCV peptides may help in the 
      understanding of the immune response to the virus. However, a panel of tetramers 
      based on several parts of the HCV polyprotein may be a mandatory requirement to 
      explore the whole breadth of the CD8(+) T-cell response against HCV and to detect 
      that response in the majority of patients with chronic infection.
FAU - Lopez-Labrador, F Xavier
AU  - Lopez-Labrador FX
AD  - Department of Medicine (Gastroenterology and Hepatology), Stanford University 
      School of Medicine, CA 94305, USA. fxlopez@teleline.es
FAU - He, Xiao-Song
AU  - He XS
FAU - Berenguer, Marina
AU  - Berenguer M
FAU - Cheung, Ramsey C
AU  - Cheung RC
FAU - Wright, Teresa L
AU  - Wright TL
FAU - Greenberg, Harry B
AU  - Greenberg HB
LA  - eng
GR  - AI40034/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA Antigens)
RN  - 0 (NS3 protein, hepatitis C virus)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Adult
MH  - Aged
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Epitopes, T-Lymphocyte/immunology
MH  - Female
MH  - HLA Antigens/*immunology
MH  - Hepacivirus/genetics/*immunology
MH  - Hepatitis C, Chronic/*immunology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - RNA/analysis
MH  - Viral Load
MH  - Viral Nonstructural Proteins/*immunology
EDAT- 2004/04/22 05:00
MHDA- 2004/06/18 05:00
CRDT- 2004/04/22 05:00
PHST- 2003/06/06 00:00 [received]
PHST- 2003/10/06 00:00 [revised]
PHST- 2004/01/22 00:00 [accepted]
PHST- 2004/04/22 05:00 [pubmed]
PHST- 2004/06/18 05:00 [medline]
PHST- 2004/04/22 05:00 [entrez]
AID - S0022175904000675 [pii]
AID - 10.1016/j.jim.2004.01.023 [doi]
PST - ppublish
SO  - J Immunol Methods. 2004 Apr;287(1-2):91-9. doi: 10.1016/j.jim.2004.01.023.