PMID- 15100384
OWN - NLM
STAT- MEDLINE
DCOM- 20050321
LR  - 20061115
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 10
IP  - 6
DP  - 2004 Jun
TI  - The peptide nucleic acids as probes for chromosomal analysis: application to 
      human oocytes, polar bodies and preimplantation embryos.
PG  - 467-72
AB  - Peptide nucleic acids (PNA) are synthetic DNA mimics based on an uncharged 
      polyamide backbone, which hybridize with complementary DNA with high affinity and 
      specificity. PNA have recently become recognized as efficient tools for in situ 
      chromosomal identification. In the present study, this new approach has been 
      tried on isolated human oocytes, polar bodies and blastomeres. Using centromeric 
      PNA probes specific for chromosomes 1, 4, 9, 16, X and Y, we tested multicolour 
      labelling PNA reaction on 27 oocytes and 23 blastomeres. Sequential PNA 
      hybridization was performed on five oocytes and combined PNA and fluorescence in 
      situ hybridization (FISH) reactions on two oocytes. Both the rates and the types 
      of abnormalities observed are in agreement with results from previous FISH 
      studies. This preliminary study indicates that PNA probes allow a reliable 
      chromosomal analysis in isolated human oocytes and blastomeres and consequently 
      might provide an interesting adjunct to FISH for diagnostic analysis.
FAU - Paulasova, P
AU  - Paulasova P
AD  - Laboratory of Assisted Reproduction, Motol Hospital, Vuvalu 84, 150 06 Praha 5, 
      Czech Republic.
FAU - Andreo, B
AU  - Andreo B
FAU - Diblik, J
AU  - Diblik J
FAU - Macek, M
AU  - Macek M
FAU - Pellestor, F
AU  - Pellestor F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040420
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (Peptide Nucleic Acids)
SB  - IM
MH  - *Blastocyst/cytology/physiology
MH  - Blastomeres/cytology/physiology
MH  - Chromosomes, Human/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Oocytes/cytology/physiology
MH  - Peptide Nucleic Acids/*metabolism
EDAT- 2004/04/22 05:00
MHDA- 2005/03/22 09:00
CRDT- 2004/04/22 05:00
PHST- 2004/04/22 05:00 [pubmed]
PHST- 2005/03/22 09:00 [medline]
PHST- 2004/04/22 05:00 [entrez]
AID - gah061 [pii]
AID - 10.1093/molehr/gah061 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 2004 Jun;10(6):467-72. doi: 10.1093/molehr/gah061. Epub 2004 Apr 
      20.