PMID- 15104282
OWN - NLM
STAT- MEDLINE
DCOM- 20040511
LR  - 20101118
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 149
IP  - 1
DP  - 2004 Feb
TI  - Karyotypic evolution pathways in medulloblastoma/primitive neuroectodermal tumor 
      determined with a combination of spectral karyotyping, G-banding, and 
      fluorescence in situ hybridization.
PG  - 44-52
AB  - Medulloblastomas (MBs) or primitive neuroectodermal tumors (PNETs) represent 
      15%-30% of pediatric brain tumors and are the most common brain tumors in 
      children; they are rare in adults. Classification of these tumors is based on 
      tissue morphology and is often controversial and problematic. Karyotypic analysis 
      of these tumors using conventional cytogenetic methods is often a difficult 
      process that may be hindered by a limited number of metaphase cells and poor 
      chromosome morphology, often leading to only partial characterization of the 
      chromosomal abnormalities. We investigated three primary human tumors and four 
      cell lines (CHO-707, DAOY, D-341, and PFSK) utilizing a combination of 
      conventional G-banding, spectral karyotyping (SKY), and fluorescence in situ 
      hybridization (FISH) techniques. A high level of intratumoral heterogeneity was 
      seen, with multiple numerical and structural chromosomal aberrations. The 
      chromosomes most frequently involved in structural aberrations were chromosomes 1 
      (14 rearrangements), 7 (9 rearrangements), and 21 (9 rearrangements). The 
      chromosomes most frequently involved in numerical aberrations were chromosomes 1, 
      12, and 13 (four cases) and chromosomes 14, 17, 19, 21, 22, and X (three cases). 
      Numerous aberrant chromosomes were characterized only with the SKY analysis, and 
      based on these findings multiple clones were identified, facilitating analysis of 
      karyotypic evolution. The most frequent evolution mechanism was via 
      polyploidization, followed by acquisition of additional numerical or structural 
      aberrations (or both); however, the results showed that the karyotypic evolution 
      process in these tumors is typically divergent and complex.
FAU - Cohen, Ninette
AU  - Cohen N
AD  - Department of Pediatric Hemato-Oncology and Institute of Hematology, The Chaim 
      Sheba Medical Center, Tel Hashomer 52621, Israel.
FAU - Betts, David R
AU  - Betts DR
FAU - Tavori, Uri
AU  - Tavori U
FAU - Toren, Amos
AU  - Toren A
FAU - Ram, Tzvi
AU  - Ram T
FAU - Constantini, Shlomi
AU  - Constantini S
FAU - Grotzer, Michael A
AU  - Grotzer MA
FAU - Amariglio, Ninette
AU  - Amariglio N
FAU - Rechavi, Gideon
AU  - Rechavi G
FAU - Trakhtenbrot, Luba
AU  - Trakhtenbrot L
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Biological Evolution
MH  - Child
MH  - *Chromosome Aberrations
MH  - *Chromosome Banding
MH  - Chromosomes, Human/*genetics
MH  - Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Medulloblastoma/*genetics
MH  - Neuroectodermal Tumors, Primitive/*genetics
MH  - Ploidies
MH  - Signal Transduction
MH  - Spectral Karyotyping
MH  - Tumor Cells, Cultured
EDAT- 2004/04/24 05:00
MHDA- 2004/05/12 05:00
CRDT- 2004/04/24 05:00
PHST- 2003/05/09 00:00 [received]
PHST- 2003/06/25 00:00 [revised]
PHST- 2003/07/07 00:00 [accepted]
PHST- 2004/04/24 05:00 [pubmed]
PHST- 2004/05/12 05:00 [medline]
PHST- 2004/04/24 05:00 [entrez]
AID - S0165460803002851 [pii]
AID - 10.1016/S0165-4608(03)00285-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2004 Feb;149(1):44-52. doi: 
      10.1016/S0165-4608(03)00285-1.