PMID- 15104296
OWN - NLM
STAT- MEDLINE
DCOM- 20040504
LR  - 20220330
IS  - 0147-5185 (Print)
IS  - 0147-5185 (Linking)
VI  - 28
IP  - 3
DP  - 2004 Mar
TI  - Serous cystic neoplasms of the pancreas: an immunohistochemical analysis 
      revealing alpha-inhibin, neuron-specific enolase, and MUC6 as new markers.
PG  - 339-46
AB  - Serous cystic neoplasms (SCNs) of the pancreas include serous microcystic adenoma 
      (SMA), serous oligocystic ill-demarcated adenoma (SOIA), solid serous adenoma 
      (SSA), von Hippel-Lindau-associated cystic neoplasm (VHL-CN), and serous 
      cystadenocarcinoma (SCC). These neoplasms are histologically similar but differ 
      in their localization, gross appearance, gender distribution, and biology. A 
      centroacinar origin is assumed but has not been proven. To clarify whether the 
      various subtypes of SCN may be distinguished from each other by marker profiles 
      that might also provide evidence of their origin, the immunoprofiles of 38 SCNs 
      (21 SMAs, 13 SOIAs, 2 VHL-CNs, 1 SSA, and 1 SCC) were defined by applying 
      antibodies against cytoskeletal, neuroendocrine, hormone receptor, and mucin 
      markers. In addition, we examined the expression of calretinin and alpha-inhibin. 
      The various types of SCN showed a very similar immunoprofile, characterized by 
      positivity for cytokeratins and neuron-specific enolase and negativity for 
      vimentin and synaptophysin. Further markers that were commonly expressed in SCNs 
      were alpha-inhibin (SMAs: 76%, SOIAs: 92%, VHL-CNs: 100%), MUC6 (SMAs: 60%, 
      SOIAs: 85%, VHL-CNs: 100%), and MUC1 (SMAs: 24%, SOIAs: 38%, VHL-CNs: 50%). 
      Western blot analysis in one SMA revealed a distinct band that stained with 
      neuron-specific enolase antiserum. Alpha-inhibin was only expressed in 4 of 11 
      acinar cell carcinomas and not in five ductal adenocarcinomas, five 
      neuroendocrine tumors, one mixed ductal-endocrine carcinoma, and one acinar cell 
      cystadenoma of the pancreas. These results suggest that, despite their biologic 
      differences, the various types of SCNs are composed of the same (or a very 
      similar) cell type and may therefore have a common direction of differentiation. 
      This notion is further supported by the finding that neuron-specific enolase, 
      alpha-inhibin, and MUC6, which may be regarded as new markers for this pancreatic 
      tumor type, were also expressed in most SCNs. Because a number of SCNs share MUC1 
      and MUC6 expression with the pancreatic centroacinar cells, the possibility of a 
      histogenetic relationship has to be considered.
FAU - Kosmahl, Markus
AU  - Kosmahl M
AD  - Department of Pathology, University of Kiel, Kiel, Germany. 
      mkosmahl@path.uni-kiel.de
FAU - Wagner, Janning
AU  - Wagner J
FAU - Peters, Katharina
AU  - Peters K
FAU - Sipos, Bence
AU  - Sipos B
FAU - Kloppel, Gunter
AU  - Kloppel G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (MUC6 protein, human)
RN  - 0 (Mucin-6)
RN  - 0 (Mucins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (inhibin-alpha subunit)
RN  - 57285-09-3 (Inhibins)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Biomarkers, Tumor/analysis
MH  - Blotting, Western
MH  - Cystadenocarcinoma, Serous/chemistry/*secondary
MH  - Cystadenoma, Serous/chemistry/*pathology
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Inhibins/analysis
MH  - Male
MH  - Middle Aged
MH  - Mucin-6
MH  - Mucins/analysis
MH  - *Neoplasm Proteins/analysis
MH  - Pancreatic Neoplasms/chemistry/*pathology
MH  - Phosphopyruvate Hydratase/analysis
MH  - von Hippel-Lindau Disease/metabolism/pathology
EDAT- 2004/04/24 05:00
MHDA- 2004/05/05 05:00
CRDT- 2004/04/24 05:00
PHST- 2004/04/24 05:00 [pubmed]
PHST- 2004/05/05 05:00 [medline]
PHST- 2004/04/24 05:00 [entrez]
AID - 10.1097/00000478-200403000-00006 [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2004 Mar;28(3):339-46. doi: 10.1097/00000478-200403000-00006.