PMID- 15104673
OWN - NLM
STAT- MEDLINE
DCOM- 20051013
LR  - 20111117
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 63
IP  - 5
DP  - 2004 May
TI  - A multicenter international evaluation of single-tube amplification protocols for 
      sequencing-based typing of HLA-DRB1 and HLA-DRB3,4,5.
PG  - 412-23
AB  - We have described previously a novel single-tube polymerase chain reaction (PCR) 
      amplification (STAmp) protocol for the efficient sequencing-based typing (SBT) of 
      human leukocyte antigen (HLA)-DRB1. The PCR amplification mix includes primers to 
      each of seven allele group-sequence motifs. We have applied this principle to the 
      simultaneous SBT of HLA-DRB3, -DRB4, and -DRB5 using locus specific primers. We 
      report here a multicenter international evaluation of the STAmp protocols 
      performed as a component of the 13th International Histocompatibility Workshop. 
      Identical amplification primer mixes, sequencing primers, and DNA were sent to 
      participating laboratories. The primer mixes contained the amplification primers 
      and the PCR buffer. Each laboratory was requested to amplify the DNA with the 
      primer mixes and perform SBT on the resulting PCR protocols, using their own 
      protocols, and return the typing results for analysis. The reported results 
      indicated that the expected sequence could be obtained with a variety of PCR 
      amplification and sequencing platforms and protocols. There were difficulties but 
      these seemed unrelated to STAmp reagents and suggest that optimal SBT results can 
      be obtained if bi-directional sequencing is performed and software is used for 
      sequence verification and editing. This indicates that SBT by STAmp can be 
      applied in many laboratories for high-throughput HLA-DRB1 and HLA-DRB3,4,5 SBT.
FAU - Sayer, D C
AU  - Sayer DC
AD  - Department of Clinical Immunology and Biochemical Genetics, Royal Perth Hospital, 
      Wellington Street, Perth, Western Australia 6000, Australia. 
      david.sayer@health.wa.gov.au
FAU - Whidborne, R
AU  - Whidborne R
FAU - De Santis, D
AU  - De Santis D
FAU - Rozemuller, E H
AU  - Rozemuller EH
FAU - Christiansen, F T
AU  - Christiansen FT
FAU - Tilanus, M G
AU  - Tilanus MG
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (DNA Primers)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (HLA-DRB3 Chains)
RN  - 0 (HLA-DRB4 Chains)
RN  - 0 (HLA-DRB5 Chains)
SB  - IM
MH  - Alleles
MH  - Artifacts
MH  - Base Sequence
MH  - Clinical Laboratory Techniques/standards
MH  - DNA Primers
MH  - HLA-DR Antigens/*genetics
MH  - HLA-DRB1 Chains
MH  - HLA-DRB3 Chains
MH  - HLA-DRB4 Chains
MH  - HLA-DRB5 Chains
MH  - Humans
MH  - International Cooperation
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/standards
MH  - Software
EDAT- 2004/04/24 05:00
MHDA- 2005/10/14 09:00
CRDT- 2004/04/24 05:00
PHST- 2004/04/24 05:00 [pubmed]
PHST- 2005/10/14 09:00 [medline]
PHST- 2004/04/24 05:00 [entrez]
AID - TAN214 [pii]
AID - 10.1111/j.0001-2815.2004.00214.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2004 May;63(5):412-23. doi: 10.1111/j.0001-2815.2004.00214.x.