PMID- 15105644
OWN - NLM
STAT- MEDLINE
DCOM- 20040603
LR  - 20190823
IS  - 0147-5185 (Print)
IS  - 0147-5185 (Linking)
VI  - 28
IP  - 5
DP  - 2004 May
TI  - Analysis of parathyroid neoplasms by interphase fluorescence in situ 
      hybridization.
PG  - 578-84
AB  - Recent studies have indicated that numerical chromosomal abnormalities, including 
      changes in cyclin D1 and p53, may be involved in parathyroid tumorigenesis. We 
      analyzed a series of parathyroid neoplasms with DNA fluorescent probes to 
      evaluate the diagnostic and prognostic utility of numerical abnormalities of 
      chromosomes 1, 6, 9, 11, 13, 15, 17, and 22 and cyclin D1 and p53 gene loci. 
      Interphase fluorescence in situ hybridization (FISH) analysis was performed on 
      paraffin-embedded tissue sections from 15 parathyroid adenomas and 18 parathyroid 
      carcinomas. Directly labeled fluorescent DNA probes for the centromere region of 
      chromosomes 1, 6, 9, 11, 15, and 17, and locus-specific probes for chromosome 22 
      and chromosome 13 and for cyclin D1 and p53 gene loci were used for dual-probe 
      hybridization. Sixty-seven percent (10 of 15) parathyroid adenomas and 78% (14 of 
      18) of parathyroid carcinomas showed chromosome gains. Seventy-three percent (11 
      of 15) of parathyroid adenomas and 33% (6 of 18) of parathyroid carcinomas showed 
      chromosome losses. Normal parathyroid tissues used as controls showed no 
      chromosomal abnormalities. Parathyroid hyperplasias averaged 1.8 gains and 0.2 
      losses per case. Parathyroid adenomas averaged 2.8 gains and 0.8 losses per case, 
      and parathyroid carcinomas averaged 3.6 gains and 0.6 losses per case. In 
      summary, chromosome abnormalities, both gains and losses, are common in 
      parathyroid adenomas and carcinomas. Parathyroid carcinomas tend to show gains of 
      more chromosome than adenomas. Chromosome 11 was the most frequent chromosome 
      loss identified in parathyroid adenomas and a frequent chromosomal gain in 
      parathyroid carcinomas. These results indicate that gain of chromosome 11 is 
      associated with more aggressive biologic behavior in parathyroid neoplasms.
FAU - Erickson, Lori A
AU  - Erickson LA
AD  - Department of Laboratory Medicine and Pathology, Anatomic Pathology, Mayo Clinic 
      and Mayo Foundation, Rochester, MN 55905, USA. erickson.lori@mayo.edu
FAU - Jalal, Syed M
AU  - Jalal SM
FAU - Harwood, Aaron
AU  - Harwood A
FAU - Shearer, Brandon
AU  - Shearer B
FAU - Jin, Long
AU  - Jin L
FAU - Lloyd, Ricardo V
AU  - Lloyd RV
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adenoma/*genetics/pathology
MH  - Carcinoma/*genetics/mortality/pathology
MH  - *Chromosome Aberrations
MH  - DNA, Neoplasm/analysis
MH  - Disease-Free Survival
MH  - Female
MH  - Genes, bcl-1
MH  - Genes, p53
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Male
MH  - Middle Aged
MH  - Parathyroid Neoplasms/*genetics/mortality/pathology
MH  - Survival Rate
EDAT- 2004/04/24 05:00
MHDA- 2004/06/04 05:00
CRDT- 2004/04/24 05:00
PHST- 2004/04/24 05:00 [pubmed]
PHST- 2004/06/04 05:00 [medline]
PHST- 2004/04/24 05:00 [entrez]
AID - 00000478-200405000-00003 [pii]
AID - 10.1097/00000478-200405000-00003 [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2004 May;28(5):578-84. doi: 10.1097/00000478-200405000-00003.