PMID- 15106876
OWN - NLM
STAT- MEDLINE
DCOM- 20041109
LR  - 20191108
IS  - 1572-3887 (Print)
IS  - 1572-3887 (Linking)
VI  - 23
IP  - 2
DP  - 2004 Feb
TI  - The natural antibody repertoire of sharks and humans recognizes the potential 
      universe of antigens.
PG  - 103-18
AB  - In ancestral sharks, a rapid emergence in the evolution of the immune system 
      occurred, giving jawed-vertebrates the necessary components for the combinatorial 
      immune response (CIR). To compare the natural antibody (NAb) repertoires of the 
      most divergent vertebrates with the capacity to produce antibodies, we isolated 
      NAbs to the same set of antigens by affinity chromatography from two species of 
      Carcharhine sharks and from human polyclonal IgG and IgM antibody preparations. 
      The activities of the affinity-purified anti-T-cell receptor (anti-TCR) NAbs were 
      compared with those of monoclonal anti-TCR NAbs that were generated from a 
      systemic lupus erythematosus patient. We report that sharks and humans, 
      representing the evolutionary extremes of vertebrate species sharing the CIR, 
      have NAbs to human TCRs, Igs, the human senescent cell antigen, and to numerous 
      retroviral antigens, indicating that essential features of the combinatorial 
      repertoire and the capacity to recognize the potential universe of antigens is 
      shared among all jawed-vertebrates.
FAU - Adelman, Miranda K
AU  - Adelman MK
AD  - Microbiology and Immunology, College of Medicine, University of Arizona, Tucson, 
      AZ 85724, USA.
FAU - Schluter, Samuel F
AU  - Schluter SF
FAU - Marchalonis, John J
AU  - Marchalonis JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Protein J
JT  - The protein journal
JID - 101212092
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens)
RN  - 0 (Autoantibodies)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Receptors, Antigen, T-Cell)
SB  - IM
CIN - Protein J. 2004 Aug;23(6):357; author reply 359-60. PMID: 15517982
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antigens/*immunology
MH  - Autoantibodies/*immunology
MH  - Chromatography, Affinity
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitope Mapping
MH  - Humans
MH  - Immune System/immunology
MH  - Immunity, Innate/*immunology
MH  - Immunoglobulin G/immunology
MH  - Immunoglobulin M/immunology
MH  - Lupus Erythematosus, Systemic/immunology
MH  - Male
MH  - Molecular Sequence Data
MH  - Receptors, Antigen, T-Cell/*immunology
MH  - Sequence Homology, Amino Acid
MH  - Sharks/*immunology
EDAT- 2004/04/27 05:00
MHDA- 2004/11/13 09:00
CRDT- 2004/04/27 05:00
PHST- 2004/04/27 05:00 [pubmed]
PHST- 2004/11/13 09:00 [medline]
PHST- 2004/04/27 05:00 [entrez]
AID - 10.1023/b:jopc.0000020077.73751.76 [doi]
PST - ppublish
SO  - Protein J. 2004 Feb;23(2):103-18. doi: 10.1023/b:jopc.0000020077.73751.76.