PMID- 15106945
OWN - NLM
STAT- MEDLINE
DCOM- 20040520
LR  - 20190816
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 78
IP  - 3
DP  - 2004 Mar
TI  - Trabecular cell expression of fibronectin and MMP-3 is modulated by aqueous humor 
      growth factors.
PG  - 653-60
AB  - We investigated the mRNA and protein expression of fibronectin and stromelysin-1 
      (matrix metalloproteinase-3, MMP-3) by trabecular cells treated with growth 
      factors present in primary and secondary aqueous humors. Serum-deprived 
      trabecular cells were incubated for 48 hr or 7 days in medium containing either 
      primary or secondary aqueous humor growth factors or in serum-free medium. We 
      extracted total RNA, performed reverse transcription-polymerase chain reaction 
      using primer pairs for fibronectin, stromelysin-1 and glyceraldehyde-3-phosphate 
      dehydrogenase (GAPDH) and quantified the products. We utilized Western blotting 
      to detect and quantify fibronectin and stromelysin-1 protein. Compared to 
      controls, expression of fibronectin mRNA by trabecular cells was increased by 50 
      and 100% after incubation in primary aqueous humor growth factors for 48 hr or 7 
      days, respectively, and 50 and 130% after incubation in secondary aqueous humor 
      growth factors. Stromelysin-1 mRNA expression was decreased by 25 and 50% after 
      incubation in primary aqueous humor growth factors for 48 hr or 7 days, 
      respectively, and 80 and 85% after incubation for 48 hr or 7 days, respectively, 
      in secondary aqueous humor growth factors. Fibronectin protein increased 3.5-fold 
      and 6-fold after incubation for 48 hr with primary or secondary aqueous humor 
      growth factors, respectively; after 7 days, the level increased 4- and 7-folds, 
      respectively. Stromelysin-1 protein was not detectable by western blotting. The 
      up-regulation of fibronectin mRNA by trabecular cells exposed to growth factors 
      present in secondary aqueous humor augmented by the down-regulation of 
      stromelysin-1 mRNA contributed to the accumulation of fibronectin. Our findings 
      open the possibility that induction of stromelysin-1 gene expression in the 
      trabecular meshwork of glaucomatous eyes could effectively reduce buildup of 
      fibronectin in the aqueous outflow pathway to decrease outflow resistance in 
      glaucomatous states of the eye.
FAU - Tripathi, Brenda J
AU  - Tripathi BJ
AD  - Department of Pathology and Microbiology, University of South Carolina School of 
      Medicine, 6439 Garners Ferry Road, Columbia, SC 29209, USA. brenda@med.sc.edu
FAU - Tripathi, Ramesh C
AU  - Tripathi RC
FAU - Chen, Jianguang
AU  - Chen J
FAU - Gotsis, Stelios
AU  - Gotsis S
FAU - Li, Junping
AU  - Li J
LA  - eng
GR  - EY-08707/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Fibronectins)
RN  - 0 (Growth Substances)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Aqueous Humor/*chemistry
MH  - Cells, Cultured
MH  - Culture Media, Serum-Free
MH  - Fibronectins/genetics/*metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Growth Substances/*pharmacology
MH  - Matrix Metalloproteinase 3/genetics/*metabolism
MH  - RNA, Messenger/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Swine
MH  - Trabecular Meshwork/*drug effects/metabolism
EDAT- 2004/04/27 05:00
MHDA- 2004/05/21 05:00
CRDT- 2004/04/27 05:00
PHST- 2004/04/27 05:00 [pubmed]
PHST- 2004/05/21 05:00 [medline]
PHST- 2004/04/27 05:00 [entrez]
AID - 10.1016/j.exer.2003.09.011 [doi]
PST - ppublish
SO  - Exp Eye Res. 2004 Mar;78(3):653-60. doi: 10.1016/j.exer.2003.09.011.