PMID- 15108305
OWN - NLM
STAT- MEDLINE
DCOM- 20041223
LR  - 20231213
IS  - 1058-8388 (Print)
IS  - 1058-8388 (Linking)
VI  - 230
IP  - 1
DP  - 2004 May
TI  - Reorganization of specific chromosomal domains and activation of silent genes in 
      plant cells acquiring pluripotentiality.
PG  - 12-22
AB  - The transition from leaf cells to protoplasts (plant cells devoid of cell walls) 
      confers pluripotentiality coupled with chromatin reorganization. Here, we sought 
      to identify remodeled chromosomal domains in Arabidopsis protoplasts by tracking 
      DNA sequences undergoing changes in DNA methylation and by identifying 
      up-regulated genes. We observed a reduction in DNA methylation at a 
      pericentromeric region of chromosome 1, and up-regulation of several members of 
      the NAC (NAM/ATAF1/CUC2) domain family, two of which are located near the 
      telomeric region of chromosome 1. Fluorescence in situ hybridization (FISH) 
      analysis demonstrated that both pericentromeric and telomeric subdomains 
      underwent chromatin decondensation. This decondensation is subdomain-specific 
      inasmuch as centromeric repeats remained largely unchanged, whereas the 18S rDNA 
      underwent condensation. Within the pericentromeric subdomain, VIP1, a gene 
      encoding a b-Zip nuclear protein required for Agrobacterium infectivity, was 
      transcriptionally activated. Overexpression of this gene in tobacco resulted in 
      growth retardation and inhibition of differentiation and shoot formation. 
      Altogether, our data indicate that acquisition of pluripotentiality involves 
      changes in DNA methylation pattern and reorganization of specific chromosomal 
      subdomains. This change leads to activation of silent genes whose products are 
      involved in acquisition or maintenance of pluripotentiality and/or the ensuing 
      fate of the cell.
CI  - Copyright 2004 Wiley-Liss, Inc.
FAU - Avivi, Yigal
AU  - Avivi Y
AD  - Department of Plant Sciences, The Weizmann Institute of Science, Rehovot, Israel.
FAU - Morad, Vered
AU  - Morad V
FAU - Ben-Meir, Hagit
AU  - Ben-Meir H
FAU - Zhao, Jing
AU  - Zhao J
FAU - Kashkush, Khalil
AU  - Kashkush K
FAU - Tzfira, Tzvi
AU  - Tzfira T
FAU - Citovsky, Vitaly
AU  - Citovsky V
FAU - Grafi, Gideon
AU  - Grafi G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Dyn
JT  - Developmental dynamics : an official publication of the American Association of 
      Anatomists
JID - 9201927
RN  - 0 (Chromatin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Blotting, Northern
MH  - Cell Lineage
MH  - Cell Nucleus/metabolism
MH  - Centromere
MH  - Chromatin/metabolism
MH  - *Chromosomes, Plant
MH  - DNA/metabolism
MH  - DNA Methylation
MH  - *Gene Silencing
MH  - *Genes, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Models, Genetic
MH  - Plant Leaves/metabolism
MH  - Plant Physiological Phenomena
MH  - Plants, Genetically Modified
MH  - Protoplasts/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Telomere
MH  - Nicotiana/genetics
MH  - Up-Regulation
EDAT- 2004/04/27 05:00
MHDA- 2004/12/24 09:00
CRDT- 2004/04/27 05:00
PHST- 2004/04/27 05:00 [pubmed]
PHST- 2004/12/24 09:00 [medline]
PHST- 2004/04/27 05:00 [entrez]
AID - 10.1002/dvdy.20006 [doi]
PST - ppublish
SO  - Dev Dyn. 2004 May;230(1):12-22. doi: 10.1002/dvdy.20006.