PMID- 15109133
OWN - NLM
STAT- MEDLINE
DCOM- 20040701
LR  - 20201208
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 58
IP  - 2-3
DP  - 2004 Mar 10
TI  - Complete sequence of RNA1 and subgenomic RNA3 of Atlantic halibut nodavirus 
      (AHNV).
PG  - 117-25
AB  - The Nodaviridae are divided into the alphanodavirus genus, which infects insects, 
      and the betanodavirus genus, which infects fishes. Betanodaviruses are the 
      causative agent of viral encephalopathy and retinopathy (VER) in a number of 
      cultivated marine fish species. The Nodaviridae are small non-enveloped RNA 
      viruses that contain a genome consisting of 2 single-stranded positivesense RNA 
      segments: RNA1 (3.1 kb), which encodes the viral part of the RNA-dependent RNA 
      polymerase (RdRp); and RNA2 (1.4 kb), which encodes the capsid protein. In 
      addition to RNA1 and RNA2, a subgenomic transcript of RNA1, RNA3, is present in 
      infected cells. We have cloned and sequenced RNA1 from the Atlantic halibut 
      Hippoglossus hippoglossus nodavirus (AHNV), and for the first time, the sequence 
      of a betanodaviral subgenomic RNA3 has been determined. AHNV RNA1 was 3100 
      nucleotides in length and contained a main open reading frame encoding a 
      polypeptide of 981 amino acids. Conservative motifs for RdRp were found in the 
      deduced amino acid sequence. RNA3 was 371 nucleotides in length, and contained an 
      open reading frame encoding a peptide of 75 amino acids corresponding to a 
      hypothetical B2 protein, although sequence alignments with the alphanodavirus B2 
      proteins showed only marginal similarities. AHNV RNA replication in the fish 
      cell-line SSN-1 (derived from striped snakehead) was analysed by Northern blot 
      analysis, which indicated that RNA3 was synthesised in large amounts (compared to 
      RNA1) at an early point in time post-infection.
FAU - Sommerset, Ingunn
AU  - Sommerset I
AD  - Institute of Marine Research, Department of Aquaculture, PO Box 1870, Nordnes, 
      5817 Bergen, Norway. ingunn.sommerset@intervet.com
FAU - Nerland, Audun H
AU  - Nerland AH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - EC 2.7.7.48 (RNA-Dependent RNA Polymerase)
SB  - IM
MH  - Alternative Splicing/*genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - DNA Primers
MH  - DNA, Complementary/genetics
MH  - Fish Diseases/*virology
MH  - *Flounder
MH  - Molecular Sequence Data
MH  - Nodaviridae/*genetics
MH  - RNA Virus Infections/*veterinary
MH  - RNA-Dependent RNA Polymerase/*genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology
EDAT- 2004/04/28 05:00
MHDA- 2004/07/02 05:00
CRDT- 2004/04/28 05:00
PHST- 2004/04/28 05:00 [pubmed]
PHST- 2004/07/02 05:00 [medline]
PHST- 2004/04/28 05:00 [entrez]
AID - 10.3354/dao058117 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2004 Mar 10;58(2-3):117-25. doi: 10.3354/dao058117.