PMID- 15109438
OWN - NLM
STAT- MEDLINE
DCOM- 20040708
LR  - 20051117
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 117
IP  - 4
DP  - 2004 Apr
TI  - Investigation of the frequency of chromosomal aneuploidy using triple 
      fluorescence in situ hybridization in 12 Chinese infertile men.
PG  - 503-6
AB  - BACKGROUND: Chromosomal aberrations are the major cause of pre- and 
      post-implantation embryo wastage and some studies suggest that half of all human 
      conceptions have a chromosomal abnormality. A chromosomal aberration in human 
      sperms is also one of the causes of failure of in vitro fertilization. This study 
      was designed to ascertain whether chromosomal aneuploidy in spermatozoa is a risk 
      factor for male infertility. METHODS: Twelve infertile men were divided into two 
      groups: 10 with oligoasthenoteratozoospermia (OAT, Group A) and two with a normal 
      semen analysis (Group B). Two normal healthy sperm donors acted as controls 
      (Group C). We used fluorescence in situ hybridization (FISH) and probes for 
      chromosomes X, Y and 18 to determine the frequency of aneuploidy. RESULTS: The 
      frequencies of spermatozoa disomy for chromosomes X, Y and 18 were 0.30% and 
      0.30%, respectively, in Group B. The percentages were not significantly different 
      from those of Group C (0.15% and 0.16%). The frequencies of nullisomy for 
      chromosomes X, Y and 18 were 0.15% and 0 for Group B, and 0 and 0.15% for Group C 
      (P > 0.05). In Group A, the incidences of disomy were 1.13% and 0.96% and the 
      frequencies of nullisomy were 1.13% and 1.60%. In these three groups, the 
      incidences of diploidy were 0.60%, 1.00%, and 0.30%, respectively. Both the 
      frequencies of disomic and nullisomic spermatozoa for chromosomes X, Y, and 18 
      and of diploid spermatozoa were significantly higher in Group A than in Groups B 
      and C. The estimated total aneuploidy rates in the sperm from the three groups 
      were 42.44%, 6.05%, and 2.59%, respectively. CONCLUSION: These results indicate 
      that chromosomal aneuploidy in spermatozoa may be a risk factor for infertility.
FAU - Zhang, Qun-Fang
AU  - Zhang QF
AD  - Laboratory of Human Reproductive Engineering, Xiangya Medical School, 
      Central-South University, Changsha 410078, China.
FAU - Lu, Guang-Xiu
AU  - Lu GX
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - *Chromosomes, Human, Pair 18
MH  - *Chromosomes, Human, X
MH  - *Chromosomes, Human, Y
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Infertility, Male/etiology/*genetics
MH  - Male
MH  - Risk Factors
EDAT- 2004/04/28 05:00
MHDA- 2004/07/09 05:00
CRDT- 2004/04/28 05:00
PHST- 2004/04/28 05:00 [pubmed]
PHST- 2004/07/09 05:00 [medline]
PHST- 2004/04/28 05:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2004 Apr;117(4):503-6.