PMID- 15110752
OWN - NLM
STAT- MEDLINE
DCOM- 20040608
LR  - 20231213
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 318
IP  - 1
DP  - 2004 May 21
TI  - High molecular mass kininogen inhibits metalloproteinases of Bothrops jararaca 
      snake venom.
PG  - 53-9
AB  - High molecular mass kininogen (HK) purified from Bothrops jararaca (Bj) plasma 
      was tested on activities of the Bj venom in vivo and in vitro. Results showed 
      that, when incubated with BjHK, the Bj venom presented inhibition on hemorrhagic, 
      edema forming, myotoxic, and coagulant activities. It is well known that 
      metalloproteinases are directly or indirectly involved in these activities. 
      Similarly, human HK inhibits the hemorrhagic effect of the Bj venom as well as 
      hemorrhagic and enzymatic effects of jararhagin, a hemorrhagic metalloproteinase 
      isolated from Bj venom. Complex between HK and jararhagin was not detected by gel 
      filtration. Nevertheless, the inhibitory effect of the hemorrhagic activity of 
      the venom was only partial when HK was pre-incubated with 0.4mM ZnCl(2) or with 
      0.45mM CaCl(2). These data suggest that the inhibitory effect depends, at least 
      partially, on the competition for ions between kininogen and metalloproteinases 
      of the venom.
FAU - de Camargo Goncalves, Luis Roberto
AU  - de Camargo Goncalves LR
AD  - Laboratorio de Fisiopatologia, Instituto Butantan, Av. Vital Brazil, 1500, 
      05503-900 Sao Paulo-SP, Brazil. lrcg@butantan.gov.br
FAU - Chudzinski-Tavassi, Ana Marisa
AU  - Chudzinski-Tavassi AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Crotalid Venoms)
RN  - 0 (Kininogens)
RN  - 0 (Platelet Aggregation Inhibitors)
RN  - 0 (Snake Venoms)
RN  - EC 2.7.3.2 (Creatine Kinase)
RN  - EC 3.4.- (Metalloproteases)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - J41CSQ7QDS (Zinc)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Blood Coagulation/drug effects
MH  - Bothrops/*metabolism
MH  - Calcium/chemistry/pharmacology
MH  - Creatine Kinase/metabolism
MH  - Crotalid Venoms/antagonists & inhibitors/metabolism/pharmacology
MH  - Drug Interactions
MH  - Edema/drug therapy
MH  - Hemorrhage/chemically induced/drug therapy
MH  - Humans
MH  - Kininogens/chemistry/metabolism/*pharmacology
MH  - Male
MH  - Metalloendopeptidases/antagonists & inhibitors/metabolism/pharmacology
MH  - Metalloproteases/*antagonists & inhibitors
MH  - Mice
MH  - Molecular Weight
MH  - Platelet Aggregation Inhibitors/metabolism/pharmacology
MH  - Rabbits
MH  - Snake Venoms/chemistry/*enzymology
MH  - Zinc/chemistry/pharmacology
MH  - Bothrops jararaca Venom
EDAT- 2004/04/28 05:00
MHDA- 2004/06/21 10:00
CRDT- 2004/04/28 05:00
PHST- 2004/03/23 00:00 [received]
PHST- 2004/04/28 05:00 [pubmed]
PHST- 2004/06/21 10:00 [medline]
PHST- 2004/04/28 05:00 [entrez]
AID - S0006291X04006990 [pii]
AID - 10.1016/j.bbrc.2004.03.182 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2004 May 21;318(1):53-9. doi: 
      10.1016/j.bbrc.2004.03.182.